Calculator
SMPEG2
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 425.39 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Crosslinkers
Molecular weight 425.39 |
SM(PEG)2 is an amine-to-sulfhydryl crosslinkers with 17.6 angstroms length. It has an amino group-reactive NHS ester and sulfhydryl group-reactive maleimide. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. SM(PEG)2 is used for irreversibly crosslinking proteins or peptides by flexible PEG spacer arms. The PEG arms help maintain conjugate solubility, reducing tendency toward aggregation and immunogenicity. It is ideal for performing controlled, two-step protein conjugations as SMCC.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of SMPEG2 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 235.078 µL | 1.175 mL | 2.351 mL | 11.754 mL | 23.508 mL |
| 5 mM | 47.016 µL | 235.078 µL | 470.157 µL | 2.351 mL | 4.702 mL |
| 10 mM | 23.508 µL | 117.539 µL | 235.078 µL | 1.175 mL | 2.351 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Chemical structure for SMPEG2.
References
View all 49 references: Citation Explorer
Cross-linking of bovine rhodopsin with sulfosuccinimidyl 4-(N maleimidomethyl)cyclohexane-1-carboxylate affects its functionality.
Authors: Medina, Rafael and Perdomo, Deisy and Möller, Carolina and Bubis, José
Journal: The Biochemical journal (2020)
Authors: Medina, Rafael and Perdomo, Deisy and Möller, Carolina and Bubis, José
Journal: The Biochemical journal (2020)
Biocompatible Organic Coatings Based on Bisphosphonic Acid RGD-Derivatives for PEO-Modified Titanium Implants.
Authors: Parfenova, Lyudmila V and Lukina, Elena S and Galimshina, Zulfia R and Gil'fanova, Guzel U and Mukaeva, Veta R and Farrakhov, Ruzil G and Danilko, Ksenia V and Dyakonov, Grigory S and Parfenov, Evgeny V
Journal: Molecules (Basel, Switzerland) (2020)
Authors: Parfenova, Lyudmila V and Lukina, Elena S and Galimshina, Zulfia R and Gil'fanova, Guzel U and Mukaeva, Veta R and Farrakhov, Ruzil G and Danilko, Ksenia V and Dyakonov, Grigory S and Parfenov, Evgeny V
Journal: Molecules (Basel, Switzerland) (2020)
Dietary Pattern Specific Protein Biomarkers for Cardiovascular Disease: A Cross-Sectional Study in 2 Independent Cohorts.
Authors: Warensjö Lemming, Eva and Byberg, Liisa and Stattin, Karl and Ahmad, Shafqat and Lind, Lars and Elmståhl, Sölve and Larsson, Susanna C and Wolk, Alicja and Michaëlsson, Karl
Journal: Journal of the American Heart Association (2019): e011860
Authors: Warensjö Lemming, Eva and Byberg, Liisa and Stattin, Karl and Ahmad, Shafqat and Lind, Lars and Elmståhl, Sölve and Larsson, Susanna C and Wolk, Alicja and Michaëlsson, Karl
Journal: Journal of the American Heart Association (2019): e011860
Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction.
Authors: Li, Jiankui and Chen, Xi and Ling, Kaijian and Liang, Zhiqing and Xu, Huicheng
Journal: Journal of biomaterials applications (2019): 808-818
Authors: Li, Jiankui and Chen, Xi and Ling, Kaijian and Liang, Zhiqing and Xu, Huicheng
Journal: Journal of biomaterials applications (2019): 808-818
Physical activity is associated with a large number of cardiovascular-specific proteins: Cross-sectional analyses in two independent cohorts.
Authors: Stattin, Karl and Lind, Lars and Elmståhl, Sölve and Wolk, Alicja and Lemming, Eva Warensjö and Melhus, Håkan and Michaëlsson, Karl and Byberg, Liisa
Journal: European journal of preventive cardiology (2019): 1865-1873
Authors: Stattin, Karl and Lind, Lars and Elmståhl, Sölve and Wolk, Alicja and Lemming, Eva Warensjö and Melhus, Håkan and Michaëlsson, Karl and Byberg, Liisa
Journal: European journal of preventive cardiology (2019): 1865-1873
Type 2 Diabetes in Relation to Hip Bone Density, Area, and Bone Turnover in Swedish Men and Women: A Cross-Sectional Study.
Authors: Mitchell, Adam and Fall, Tove and Melhus, Håkan and Wolk, Alicja and Michaëlsson, Karl and Byberg, Liisa
Journal: Calcified tissue international (2018): 501-511
Authors: Mitchell, Adam and Fall, Tove and Melhus, Håkan and Wolk, Alicja and Michaëlsson, Karl and Byberg, Liisa
Journal: Calcified tissue international (2018): 501-511
Antitumor activity and carrier properties of novel hemocyanins coupled to a mimotope of GD2 ganglioside.
Authors: Palacios, Miriam and Tampe, Ricardo and Del Campo, Miguel and Zhong, Ta-Ying and López, Mercedes N and Salazar-Onfray, Flavio and Becker, María Inés
Journal: European journal of medicinal chemistry (2018): 74-86
Authors: Palacios, Miriam and Tampe, Ricardo and Del Campo, Miguel and Zhong, Ta-Ying and López, Mercedes N and Salazar-Onfray, Flavio and Becker, María Inés
Journal: European journal of medicinal chemistry (2018): 74-86
Impacts of Cross-Linkers on Biological Effects of Mesoporous Silica Nanoparticles.
Authors: Chen, Yi-Ping and Wu, Si-Han and Chen, I-Chih and Chen, Chien-Tsu
Journal: ACS applied materials & interfaces (2017): 10254-10265
Authors: Chen, Yi-Ping and Wu, Si-Han and Chen, I-Chih and Chen, Chien-Tsu
Journal: ACS applied materials & interfaces (2017): 10254-10265
Structural Characterization of Cross-Linked Species in Trastuzumab Emtansine (Kadcyla).
Authors: Chen, Yan and Kim, Michael T and Zheng, Laura and Deperalta, Galahad and Jacobson, Fred
Journal: Bioconjugate chemistry (2016): 2037-47
Authors: Chen, Yan and Kim, Michael T and Zheng, Laura and Deperalta, Galahad and Jacobson, Fred
Journal: Bioconjugate chemistry (2016): 2037-47
Enhanced tumor targeting of cRGD peptide-conjugated albumin nanoparticles in the BxPC-3 cell line.
Authors: Yu, Xinzhe and Song, Yunlong and Di, Yang and He, Hang and Fu, Deliang and Jin, Chen
Journal: Scientific reports (2016): 31539
Authors: Yu, Xinzhe and Song, Yunlong and Di, Yang and He, Hang and Fu, Deliang and Jin, Chen
Journal: Scientific reports (2016): 31539
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
Fluorescent Oligonucleotide Labeling Reagents
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
Fluorescent Oligonucleotide Labeling Reagents