Calculator
SMPEG4
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 513.50 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Crosslinkers
Molecular weight 513.50 |
SM(PEG)4 is an amine-to-sulfhydryl crosslinkers with 24.6 angstroms length. It has an amino group-reactive NHS ester and sulfhydryl group-reactive maleimide. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. SM(PEG)4 is used for irreversibly crosslinking proteins or peptides by flexible PEG spacer arms. The PEG arms help maintain conjugate solubility, reducing tendency toward aggregation and immunogenicity. It is ideal for performing controlled, two-step protein conjugations as SMCC.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of SMPEG4 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 194.742 µL | 973.71 µL | 1.947 mL | 9.737 mL | 19.474 mL |
| 5 mM | 38.948 µL | 194.742 µL | 389.484 µL | 1.947 mL | 3.895 mL |
| 10 mM | 19.474 µL | 97.371 µL | 194.742 µL | 973.71 µL | 1.947 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Chemical structure for SMPEG4.
References
View all 50 references: Citation Explorer
Tertiary blends of PAMAM/PEG/PEG tissue bioadhesives.
Authors: Shah, Ankur Harish and Djordjevic, Ivan and Steele, Terry W J
Journal: Journal of the mechanical behavior of biomedical materials (2020): 103405
Authors: Shah, Ankur Harish and Djordjevic, Ivan and Steele, Terry W J
Journal: Journal of the mechanical behavior of biomedical materials (2020): 103405
Enhanced regeneration of osteochondral defects by using an aggrecanase-1 responsively degradable and N-cadherin mimetic peptide-conjugated hydrogel loaded with BMSCs.
Authors: Feng, Xue and Zhou, Tong and Xu, Peifang and Ye, Juan and Gou, Zhongru and Gao, Changyou
Journal: Biomaterials science (2020): 2212-2226
Authors: Feng, Xue and Zhou, Tong and Xu, Peifang and Ye, Juan and Gou, Zhongru and Gao, Changyou
Journal: Biomaterials science (2020): 2212-2226
Crosslinker length dictates step-growth hydrogel network formation dynamics and allows rapid on-chip photoencapsulation.
Authors: Jiang, Zhongliang and Shaha, Rajib and McBride, Ralph and Jiang, Kun and Tang, Mingchen and Xu, Bang and Goroncy, Alexander K and Frick, Carl and Oakey, John
Journal: Biofabrication (2020): 035006
Authors: Jiang, Zhongliang and Shaha, Rajib and McBride, Ralph and Jiang, Kun and Tang, Mingchen and Xu, Bang and Goroncy, Alexander K and Frick, Carl and Oakey, John
Journal: Biofabrication (2020): 035006
Cell encapsulation spatially alters crosslink density of poly(ethylene glycol) hydrogels formed from free-radical polymerizations.
Authors: Chu, Stanley and Maples, Mollie M and Bryant, Stephanie J
Journal: Acta biomaterialia (2020): 37-50
Authors: Chu, Stanley and Maples, Mollie M and Bryant, Stephanie J
Journal: Acta biomaterialia (2020): 37-50
A triply modified human adult hemoglobin with low oxygen affinity, rapid autoxidation and high tetramer stability.
Authors: Yan, Wenying and Shen, Lijuan and Yu, Weili and Wang, Ying and Wang, Quan and You, Guoxing and Zhao, Lian and Zhou, Hong and Hu, Tao
Journal: International journal of biological macromolecules (2020): 236-242
Authors: Yan, Wenying and Shen, Lijuan and Yu, Weili and Wang, Ying and Wang, Quan and You, Guoxing and Zhao, Lian and Zhou, Hong and Hu, Tao
Journal: International journal of biological macromolecules (2020): 236-242
A comparison of human mesenchymal stem cell osteogenesis in poly(ethylene glycol) hydrogels as a function of MMP-sensitive crosslinker and crosslink density in chemically defined medium.
Authors: Aziz, Aaron H and Bryant, Stephanie J
Journal: Biotechnology and bioengineering (2019): 1523-1536
Authors: Aziz, Aaron H and Bryant, Stephanie J
Journal: Biotechnology and bioengineering (2019): 1523-1536
Coaxial PCL/PEG-thiol-ene microfiber with tunable physico-chemical properties for regenerative scaffolds.
Authors: Iglesias-Echevarria, Monica and Durante, Luca and Johnson, Richard and Rafuse, Michael and Ding, Yonghui and Bonani, Walter and Maniglio, Devid and Tan, Wei
Journal: Biomaterials science (2019): 3640-3651
Authors: Iglesias-Echevarria, Monica and Durante, Luca and Johnson, Richard and Rafuse, Michael and Ding, Yonghui and Bonani, Walter and Maniglio, Devid and Tan, Wei
Journal: Biomaterials science (2019): 3640-3651
Fabrication, characterization, and implementation of engineered hydrogels for controlling breast cancer cell phenotype and dormancy.
Authors: Pradhan, Shantanu and Slater, John H
Journal: MethodsX (2019): 2744-2766
Authors: Pradhan, Shantanu and Slater, John H
Journal: MethodsX (2019): 2744-2766
Dynamic control of hydrogel crosslinking via sortase-mediated reversible transpeptidation.
Authors: Arkenberg, Matthew R and Moore, Dustin M and Lin, Chien-Chi
Journal: Acta biomaterialia (2019): 83-95
Authors: Arkenberg, Matthew R and Moore, Dustin M and Lin, Chien-Chi
Journal: Acta biomaterialia (2019): 83-95
Multi-component supramolecular fibers with elastomeric properties and controlled drug release.
Authors: Putti, Matilde and Mes, Tristan and Huang, Jingyi and Bosman, Anton W and Dankers, Patricia Y W
Journal: Biomaterials science (2019): 163-173
Authors: Putti, Matilde and Mes, Tristan and Huang, Jingyi and Bosman, Anton W and Dankers, Patricia Y W
Journal: Biomaterials science (2019): 163-173
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
Fluorescent Oligonucleotide Labeling Reagents
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
Fluorescent Oligonucleotide Labeling Reagents