Name: Spexyte™ Micro pH Probe *Optimized for Nanodrop*
Brand: AAT Bioquest
SKU: 21230
Price: 115 USD
Availability: InStock

Spexyte™ Micro pH Probe

Optimized for Nanodrop

Biological samples are usually in a very narrow pH range from 4.5 to 8.5, and many of them need to be provided in micro-volume (e.g., microliter). It is difficult to measure the pH values with small volumes accurately using the conventional pH meters due to the bulky sizes of the conventional pH electrodes. Spexyte™ Micro pH Probe is optimized to measure µL samples in the range of pH 4.5 ~8.5 using a NanoDrop™ or an equivalent device. The pH value is measured by reading the ratio of BCFL absorbances at 502 nm to 470 nm since the relationship between the pH value and the absorbance ratio (502/470nm) fits the Four-parameter logistic model well. The pH of a microvolume biological solution could be measured quickly and accurately in seconds with Spexyte™ Micro pH Probe. It provides a sensitive, easy and fast method to measure the pH values for micro-volume samples.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21230	2x250 Tests	Price

Citations

View all 2 citations: Citation Explorer

The F0F1 ATP synthase regulates human neutrophil migration through cytoplasmic proton extrusion coupled with ATP generation

Authors:

Gao, Jun and Zhang, Tian and Kang, Zhanfang and Ting, Weijen and Xu, Lingqing and Yin, Dazhong

Journal:

Molecular Immunology (2017): 219--226

Oxidative Stress-Activated NHE1 Is Involved in High Glucose-Induced Apoptosis in Renal Tubular Epithelial Cells

Authors:

Wu, Yiqing and Zhang, Min and Liu, Rui and Zhao, Chunjie

Journal:

Yonsei Medical Journal (2016): 1252--1259

References

View all 57 references: Citation Explorer

PHD2 is a regulator for glycolytic reprogramming in macrophages

Authors:

Guentsch, Annemarie and Beneke, Angelika and Swain, Lija and Farhat, Katja and Nagarajan, Shunmugam and Wielockx, Ben and Raithatha, Kaamini and Dudek, Jan and Rehling, Peter and Zieseniss, Anke and others, undefined

Journal:

Molecular and Cellular Biology (2016): MCB--00236

Monitoring phospholipid dynamics during phagocytosis: application of genetically-encoded fluorescent probes

Authors:

Sarantis H, Grinstein S.

Journal:

Methods Cell Biol (2012): 429

Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock

Authors:

Schreiner L, Huber-Lang M, Weiss ME, Hohmann H, Schmolz M, Schneider EM.

Journal:

J Cell Commun Signal (2011): 135

The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis

Authors:

Flannagan RS, Grinstein S.

Journal:

Methods Mol Biol (2010): 121

Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms

Authors:

Leclerc L, Boudard D, Pourchez J, Forest V, Sabido O, Bin V, Palle S, Grosseau P, Bernache D, Cottier M.

Journal:

Inhal Toxicol (2010): 1091

Physical properties

Molecular weight	N/A
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Fax
Email	sales@aatbio.com
International	See distributors
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Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025