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SPQ [6-Methoxy-N-(3-sulfopropyl)quinolinium]

A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of SPQ [6-Methoxy-N-(3-sulfopropyl)quinolinium] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM355.454 µL1.777 mL3.555 mL17.773 mL35.545 mL
5 mM71.091 µL355.454 µL710.909 µL3.555 mL7.109 mL
10 mM35.545 µL177.727 µL355.454 µL1.777 mL3.555 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 71 references: Citation Explorer
Expression of band 3 protein on erythrocytes of malignant tumor patients and its impact on proliferation of K562 cells
Authors: Bai SZ, Liu XB, Xi YH, Wang TY, Fu GH.
Journal: Ai Zheng (2005): 543
Measurement of halide efflux from cultured and primary airway epithelial cells using fluorescence indicators
Authors: Munkonge F, Alton EW, Andersson C, Davidson H, Dragomir A, Edelman A, Farley R, Hjelte L, McLachlan G, Stern M, Roomans GM.
Journal: J Cyst Fibros (2004): 171
Halide fluxes in epithelial cells measured with an automated cell plate reader
Authors: Mahlangu DA, Dix JA.
Journal: Anal Biochem (2004): 28
Purinergic signaling underlies CFTR control of human airway epithelial cell volume
Authors: Braunstein GM, Zsembery A, Tucker TA, Schwiebert EM.
Journal: J Cyst Fibros (2004): 99
Normal function of the cystic fibrosis conductance regulator protein can be associated with homozygous (Delta)F508 mutation
Authors: Sermet-Gaudelus I, Vallee B, Urbin I, Torossi T, Marianovski R, Fajac A, Feuillet MN, Bresson JL, Lenoir G, Bernaudin JF, Edelman A.
Journal: Pediatr Res (2002): 628
Page updated on November 9, 2024

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Physical properties

Molecular weight

281.33

Solvent

DMSO

Spectral properties

Excitation (nm)

317

Emission (nm)

451

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

83907-40-8
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