Name: SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]
Brand: AAT Bioquest
SKU: 13472
Availability: InStock

SRB-VAD-FMK

Sulforhodamine B-VAD-FMK

SRB-VAD is a red fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the SRB-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry. The probe has the spectral properties similar to Cy3® and TRITC, thus the filter set of Cy3® and TRITC can be conveniently used with a flow cytometer or microscope.

Protocol

COA

Catalog	Size	Price	Quantity
13472	25 Tests	Price
13482	100 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of SRB-VAD-FMK [Sulforhodamine B-VAD-FMK] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	114.415 µL	572.076 µL	1.144 mL	5.721 mL	11.442 mL
5 mM	22.883 µL	114.415 µL	228.83 µL	1.144 mL	2.288 mL
10 mM	11.442 µL	57.208 µL	114.415 µL	572.076 µL	1.144 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	874.01 g/mol	=	mL	x	mM	=	mmol

References

View all 26 references: Citation Explorer

Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties

Authors:

Lawrence CP, Chow SC.

Journal:

Toxicol Appl Pharmacol. (2012)

Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice

Authors:

Li YY, Yan CL.

Journal:

Zhonghua Yi Xue Za Zhi (2012): 1992

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death

Authors:

Meslin B, Beavogui AH, Fasel N, Picot S.

Journal:

PLoS One (2011): e23867

Physical properties

Molecular weight	874.01
Solvent	DMSO

Spectral properties

Absorbance (nm)	220
Excitation (nm)	559
Emission (nm)	577

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet (Catalog 13472)
Safety Data Sheet (Catalog 13482)
Certificate of Analysis

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Page updated on October 24, 2025