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Stain-All *CAS 7423-31-6*

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Physical properties
Molecular weight559.58
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
Stains-all is suitable for differential staining of nucleic acids and proteins. It stains RNA on bluish purple, DNA in blue and proteins in red. As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Example protocol


Store at -20 °C. Expiration date is one year from the date of receipt.

Maximum Excitation: 573 nm
Maximum Emission: 609 nm


Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. 0.1% Stains-All stock stain:
Add 10 mg of Stains-All into 10 mL of formamide and mix well.


Stains-All working solution:
Mix 1 mL stock stain, 1 mL formamide, 5 mL isopropranol, 100 μL Tris-HCl (3.0 M, pH-8.8) and 12.9 mL water, Mix well.


Table 1. Stain colors of different biomolecules with Stain-All

BiomoleculeStain Color
RNABluish Purple
Acid MucopolysaccaridesVarious

Gel Electrophoresis

  1. Stain gel by soaking it in a covered container for 18 to 24 hours, protected from light.

  2. Destain the gel by soaking it in water, protected from light. Note: Exposure to light will produce a dull yellow background. Note: Destaining may also be done by exposing the gel to light for 30 minutes.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Stain-All *CAS 7423-31-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM178.705 µL893.527 µL1.787 mL8.935 mL17.871 mL
5 mM35.741 µL178.705 µL357.411 µL1.787 mL3.574 mL
10 mM17.871 µL89.353 µL178.705 µL893.527 µL1.787 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



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