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Stain-All *CAS 7423-31-6*
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Application notes
Stains-all is suitable for differential staining of nucleic acids and proteins. It stains RNA on bluish purple, DNA in blue and proteins in red. As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.
Example protocol
AT A GLANCE
Important
Store at -20 °C. Expiration date is one year from the date of receipt.
Maximum Excitation: 573 nm
Maximum Emission: 609 nm
PREPARATION OF STOCK SOLUTION
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1. 0.1% Stains-All stock stain:
Add 10 mg of Stains-All into 10 mL of formamide and mix well.
PREPARATION OF WORKING SOLUTION
Stains-All working solution:
Mix 1 mL stock stain, 1 mL formamide, 5 mL isopropranol, 100 μL Tris-HCl (3.0 M, pH-8.8) and 12.9 mL water, Mix well.
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. Stain colors of different biomolecules with Stain-All
| Biomolecule | Stain Color |
| RNA | Bluish Purple |
| DNA | Blue |
| Proteins | Red |
| Acid Mucopolysaccarides | Various |
Gel Electrophoresis
- Stain gel by soaking it in a covered container for 18 to 24 hours, protected from light.
- Destain the gel by soaking it in water, protected from light. Note: Exposure to light will produce a dull yellow background. Note: Destaining may also be done by exposing the gel to light for 30 minutes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Stain-All *CAS 7423-31-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 178.705 µL | 893.527 µL | 1.787 mL | 8.935 mL | 17.871 mL |
| 5 mM | 35.741 µL | 178.705 µL | 357.411 µL | 1.787 mL | 3.574 mL |
| 10 mM | 17.871 µL | 89.353 µL | 178.705 µL | 893.527 µL | 1.787 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Citations
View all 4 citations: Citation Explorer
DNA Ion Detector and Logic Circulation Amplification Model Based on Mercury and Silver Ions
Authors: Guo, Bingjie and Wang, Luhui and Wu, Tao and Dong, Yafei
Journal: Fundamenta Informaticae (2019): 195--205
Authors: Guo, Bingjie and Wang, Luhui and Wu, Tao and Dong, Yafei
Journal: Fundamenta Informaticae (2019): 195--205
Multifunctional Biosensor Logic Gates Based on Graphene Oxide
Authors: Wang, Luhui and Zhang, Yingying and Wei, Yani and Dong, Yafei
Journal: (2018): 473--483
Authors: Wang, Luhui and Zhang, Yingying and Wei, Yani and Dong, Yafei
Journal: (2018): 473--483
An ions-medicated single molecular multi-functional DNA cascade logic circuit and signal amplifier model
Authors: Guo, Bingjie and Chen, Xiangxiang and Wu, Tao and Dong, Yafei
Journal: (2017): 436--445
Authors: Guo, Bingjie and Chen, Xiangxiang and Wu, Tao and Dong, Yafei
Journal: (2017): 436--445
An Ions-Medicated Single Molecular Multi-functional DNA Cascade Logic Circuit and Signal Amplifier Model
Authors: Guo, Bingjie and Chen, Xiangxiang and Wu, Tao and Dong, Yafei
Journal: (2017): 436--445
Authors: Guo, Bingjie and Chen, Xiangxiang and Wu, Tao and Dong, Yafei
Journal: (2017): 436--445
References
View all 12 references: Citation Explorer
Rapid test for distinguishing membrane-active antibacterial agents
Authors: Prakash Singh M., undefined
Journal: J Microbiol Methods (2006): 125
Authors: Prakash Singh M., undefined
Journal: J Microbiol Methods (2006): 125
Stability, homodimerization, and calcium-binding properties of a single, variant betagamma-crystallin domain of the protein absent in melanoma 1 (AIM1)
Authors: Rajini B, Graham C, Wistow G, Sharma Y.
Journal: Biochemistry (2003): 4552
Authors: Rajini B, Graham C, Wistow G, Sharma Y.
Journal: Biochemistry (2003): 4552
Purification of myristoylated and nonmyristoylated neuronal calcium sensor-1 using single-step hydrophobic interaction chromatography
Authors: Fisher JR, Sharma Y, Iuliano S, Piccioti RA, Krylov D, Hurley J, Roder J, Jeromin A.
Journal: Protein Expr Purif (2000): 66
Authors: Fisher JR, Sharma Y, Iuliano S, Piccioti RA, Krylov D, Hurley J, Roder J, Jeromin A.
Journal: Protein Expr Purif (2000): 66
Comparative characteristics of sarcoplasmic reticulum preparations from skeletal muscles of the ground squirrel Spermophilus undulatus, rats, and rabbits
Authors: Shutova AN, Storey KB, Lopina OD, Rubtsov AM.
Journal: Biochemistry (Mosc) (1999): 1250
Authors: Shutova AN, Storey KB, Lopina OD, Rubtsov AM.
Journal: Biochemistry (Mosc) (1999): 1250
Slit-scan flow cytometry for consistent high resolution DNA analysis of X- and Y-chromosome bearing sperm
Authors: Rens W, Welch GR, Houck DW, van Oven CH, Johnson LA.
Journal: Cytometry (1996): 191
Authors: Rens W, Welch GR, Houck DW, van Oven CH, Johnson LA.
Journal: Cytometry (1996): 191