Name: Stayright™ Purple HRP Staining Kit
Brand: AAT Bioquest
SKU: 45905
Availability: InStock

Stayright™ Purple HRP Staining Kit

3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. However, DAB has been shown to be mutagenic and hazardous to laboratory workers and the environment. In order to address this issue, AAT provides Stayright™ Purple as a significantly safer IHC chromogen than DAB. Furthermore, Stayright™ Purple provides a rapid and simple method to develop clean and intense purple stain in the presence of HRP with high sensitivity as DAB. The Stayright™ Purple HRP substrate also shows non-mutagenic effects with minimal cytotoxicity. ReadiUse™ Stayright™ Purple Peroxidase (HRP) Substrate is suitable for use in peroxidase (HRP)-based immunohistochemistry (IHC) and in situ hybridization (ISH) staining methods. Upon HRP-induced oxidation, Stayright™ Purple forms a purple insoluble precipitating product at the target site of your assay. The purple end product is insoluble in organic solvents and organic mounting media, thus the distinct purple stain can maintain through regular dehydration and coverslipping steps. For enhanced convenience, you try our ReadiUse™ Stayright™ Purple Peroxidase (HRP) Substrate (#45900 and 45901). It is a stable pre-mixed solution containing hydrogen peroxide so all mixing steps are eliminated and is ready to use.

Immunohistochemical detection of EpCAM in FFPE lung adenocarcinoma tissue. The tissue sections were incubated with poly-HRP conjugated Goat anti-Rabbit IgG and then developed with Stayright™ Purple (Left) or DAB (Right), respectively. Cells were also counterstained with hematoxylin. Stayright™ Purple generates an intense stain with high sensitivity and clear resolution similar as DAB.

Protocol

COA

Catalog	Size	Price	Quantity
45905	5 mL	Price
45906	50 mL	Price

Citations

View all 27 citations: Citation Explorer

Involvement of lncRNA MIR205HG in idiopathic pulmonary fibrosis and IL-33 regulation via Alu elements

Authors:

Takashima, Tsuyoshi and Zeng, Chao and Murakami, Eitaro and Fujiwara, Naoko and Kohara, Masaharu and Nagata, Hideki and Feng, Zhaozu and Sugai, Ayako and Harada, Yasue and Ichijo, Rika and others,

Journal:

JCI Insight (2025)

A pontine-medullary loop crucial for REM sleep and its deficit in Parkinson’s disease

Authors:

Kashiwagi, Mitsuaki and Beck, Goichi and Kanuka, Mika and Arai, Yoshifumi and Tanaka, Kaeko and Tatsuzawa, Chika and Koga, Yumiko and Saito, Yuki C and Takagi, Marina and Oishi, Yo and others,

Journal:

Cell (2024)

3,3'-diaminobenzidine (DAB)-H2O2-HRP voltammetric enzyme-linked immunoassay for the detection of carcionembryonic antigen

Authors:

Zhang, S., Yang, J., Lin, J.

Journal:

Bioelectrochemistry (2008): 47-52

HistoGreen: a new alternative to 3,3'-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) as a peroxidase substrate in immunohistochemistry?

Authors:

Thomas, M. A., Lemmer, B.

Journal:

Brain Res Brain Res Protoc (2005): 107-18

Stability and solubility of 3,3'-diaminobenzidine (DAB)

Authors:

Kiernan, J. A.

Journal:

Biotech Histochem (2003): 135

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Light microscope
Instrument specification(s)	White light

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Page updated on September 30, 2025

Component A: 100X Stayright&#x2122 Purple	1 bottle (50 &#x03bcL)
Component B: Stayright&#x2122 Purple HRP buffer	1 bottle (5 mL)
Component C: Stabilized 3% Hydrogen peroxide (H2O2)	1 vial (10 &#x03bcL)

Component A: 100X Stayright&#x2122 Purple	1 bottle (500 &#x03bcL)
Component B: Stayright&#x2122 Purple HRP buffer	1 bottle (50 mL)
Component C: Stabilized 3% Hydrogen peroxide (H2O2)	1 bottle (100 &#x03bcL)