Stayright™ Purple HRP Staining Kit

3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. However, DAB has been shown to be mutagenic and hazardous to laboratory workers and the environment. In order to address this issue, AAT provides Stayright™ Purple as a significantly safer IHC chromogen than DAB. Furthermore, Stayright™ Purple provides a rapid and simple method to develop clean and intense purple stain in the presence of HRP with high sensitivity as DAB. The Stayright™ Purple HRP substrate also shows non-mutagenic effects with minimal cytotoxicity. ReadiUse™ Stayright™ Purple Peroxidase (HRP) Substrate is suitable for use in peroxidase (HRP)-based immunohistochemistry (IHC) and in situ hybridization (ISH) staining methods. Upon HRP-induced oxidation, Stayright™ Purple forms a purple insoluble precipitating product at the target site of your assay. The purple end product is insoluble in organic solvents and organic mounting media, thus the distinct purple stain can maintain through regular dehydration and coverslipping steps. For enhanced convenience, you try our ReadiUse™ Stayright™ Purple Peroxidase (HRP) Substrate (#45900 and 45901). It is a stable pre-mixed solution containing hydrogen peroxide so all mixing steps are eliminated and is ready to use.