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Streptavidin-iFluor 750®-xtra conjugate
Product key features
Streptavidin-iFluor 750®-xtra Conjugate is a near-infrared fluorescent probe for detecting biotinylated targets.
- High Sensitivity: iFluor® 750-xtra provides a stable NIR fluorescence for low-abundance target detection.
- Specific Biotin Binding: Streptavidin binds biotin with high affinity.
- Direct equivalent: Replacement for Streptavidin-Alexa Fluor® 750 from Thermo Fisher.
- Versatile Applications: Suitable for Western blot, microscopy, and in vivo imaging.
Product description
Streptavidin-iFluor 750®-xtra Conjugate is a highly sensitive fluorescent reagent designed for detecting biotinylated biomolecules with near-infrared fluorescence. It features a streptavidin core for strong, specific binding to biotin, and is conjugated to iFluor® 750, a photostable fluorophore with excitation/emission maxima around 755/778 nm.
The near-infrared emission of iFluor® 750-xtra minimizes background autofluorescence interference and avoids spectral overlap with visible-range dyes, making it ideal for multicolor fluorescence detection in microscopy, flow cytometry, Western blotting, and in vivo imaging.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| APC-iFluor® 750-streptavidin conjugate | 651 | 793 | 700000 |
| RPE-iFluor® 750-streptavidin conjugate | 565 | 778 | 1960000 |
References
View all 8 references: Citation Explorer
Fluorescence In Situ Hybridization of Small Non-Coding RNAs.
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Tricellulin Expression and its Deletion Effects in the Endolymphatic Sac.
Authors: Matsubara, Ai and Miyashita, Takenori and Inamoto, Ryuhei and Sakaguchi, Hirofumi and Kamitani, Toru and Mori, Nozomu and Hoshikawa, Hiroshi
Journal: The journal of international advanced otology (2018): 312-316
Authors: Matsubara, Ai and Miyashita, Takenori and Inamoto, Ryuhei and Sakaguchi, Hirofumi and Kamitani, Toru and Mori, Nozomu and Hoshikawa, Hiroshi
Journal: The journal of international advanced otology (2018): 312-316
Plasmonic activation of gold nanorods for remote stimulation of calcium signaling and protein expression in HEK 293T cells.
Authors: Sanchez-Rodriguez, Sandra P and Sauer, Jeremy P and Stanley, Sarah A and Qian, Xi and Gottesdiener, Andrew and Friedman, Jeffrey M and Dordick, Jonathan S
Journal: Biotechnology and bioengineering (2016): 2228-40
Authors: Sanchez-Rodriguez, Sandra P and Sauer, Jeremy P and Stanley, Sarah A and Qian, Xi and Gottesdiener, Andrew and Friedman, Jeffrey M and Dordick, Jonathan S
Journal: Biotechnology and bioengineering (2016): 2228-40
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