(Suc-LLVY)2R110

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Chemical structure for (Suc-LLVY)2R110
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Unit Size: Cat No: Price (USD): Qty:
1 mg 13451 $95


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Overview

Ex/Em (nm)498/520
MW1507.72
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
InstrumentsFluorescence microplate reader
Category Enzyme Detection
Peptidases and Proteases
Related Secondary Reagents
(Suc-LLVY)2R110 is a sentive fluorogenic substrate for 20S proteasome, calpains and other chymotrypsin-like proteases. The non-fluorescent substrate generates a bright green fluorescent rhodamine 110 product that has Ex/Em = 494/521 nm, and can be easily detected with a FITC filter set. This rhodamine 110 substrate is much more sensitive than the AMC-, AFC- or 4-nitroaniline-based substrates. The most common form of the proteasome is known as the 26S proteasome that contains one 20S core particle structure and two 19S regulatory caps. All 20S particles consist of four stacked heptameric ring structures that are themselves composed of two different types of subunits; alpha subunits are structural in nature, whereas beta subunits are predominantly catalytic. The outer two rings in the stack consist of seven alpha subunits each, which serve as docking domains for the regulatory particles and the alpha subunits N-termini form a gate that blocks unregulated access of substrates to the interior cavity. The inner two rings each consist of seven beta subunits and contain the protease active sites that perform the proteolysis reactions.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of (Suc-LLVY)2R110 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

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Wavelength (nm)





Protocol


Quick Preview

This protocol only provides a guideline, and should be modified according to your specific needs.
At a glance

Important notes
Following protocol only provides a guideline, and should be modified according to your specific needs.

Product Number

Indicators

Excitation

Emission

13451

(Suc-LLVY)2R110

498 nm

520 nm

13453

Suc-LLVY-AMC

351 nm

430 nm

13455

(Ac-ANW)2R110

498 nm

520 nm

13465

(Ac-KQL)2R110

498 nm

520 nm

13466

(Z-LLE)2R110

498 nm

520 nm

13467

(Ac-PAL)2R110

498 nm

520 nm

13468

(Ac-WLA)2R110

498 nm

520 nm

Key parameters
Instrument:Fluorescence microplate reader
Excitation:498 nm
Emission:520 nm
Recommended plate:Solid black
Preparation of stock solution
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. (Suc-LLVY)2R110 stock solution:
Prepare a 5 to 10 mM stock solution in DMSO.

Preparation of working solution


ComponentVolume
Substrate stock solution (10 mM)25 µL
DTT (1M)100 µL
EDTA (100 mM)400 µL
Hepes Buffer (25 mM), pH = 7.410 mL
 
Total volume10.53 mL
Sample experimental protocol
  1. Mix equal volume of the protesome standards or samples with 2X fluorescent proteasome substrate assay solution, and incubate the solutions at room temperature for at least 1 hour.

  2. Monitor the fluorescence using fluorescent microplate readers at 498/520 nm.
Example data analysis and figures

Figure 1. Chemical structure for (Suc-LLVY)2R110
Disclaimer
AAT Bioquest provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please consult the Safety Data Sheet (SDS) provided for the product. Chemical analysis and/or reverse engineering of any kit or its components is strictly prohibited without written permission from AAT Bioquest. Please call 408-733-1055 or email info@aatbio.com if you have any questions.





References & Citations

Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Wenjuan Zhou, Lingling Wei, Ting Xiao, Chunyou Lai, Min Peng, Lingli Xu, Xiangwei Luo, Shaoping Deng, Fengxue Zhang
Journal: Biochemical and Biophysical Research Communications (2017): 400--406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Jennifer E Gilda, Xianyin Lai, Frank A Witzmann, Aldrin V Gomes
Journal: Molecular & Cellular Proteomics (2016): 1962--1981

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Rajeshwary Ghosh, Sumanta K Goswami, Luis Felipe BB Feitoza, Bruce Hammock, Aldrin V Gomes
Journal: International Journal of Cardiology (2016): 923--935

Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1
Authors: Stefanie Grimm, Christiane Ott, Melanie Hörlacher, Daniela Weber, Annika Höhn, Tilman Grune
Journal: Biochemical Journal (2012): 127--139






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Enzyme Probes & Assay Kits

Certificate of Analysis