Name: Suc-LLVY-AMC
Brand: AAT Bioquest
SKU: 13453
Price: 115 USD
Availability: InStock

Suc-LLVY-AMC

Suc-LLVY-AMC is a sentive fluorogenic substrate for 20S proteasome, calpains and other chymotrypsin-like proteases. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. The most common form of the proteasome is known as the 26S proteasome that contains one 20S core particle structure and two 19S regulatory caps. All 20S particles consist of four stacked heptameric ring structures that are themselves composed of two different types of subunits; alpha subunits are structural in nature, whereas beta subunits are predominantly catalytic. The outer two rings in the stack consist of seven alpha subunits each, which serve as docking domains for the regulatory particles and the alpha subunits N-termini form a gate that blocks unregulated access of substrates to the interior cavity. The inner two rings each consist of seven beta subunits and contain the protease active sites that perform the proteolysis reactions.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13453	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Suc-LLVY-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	126.272 µL	631.361 µL	1.263 mL	6.314 mL	12.627 mL
5 mM	25.254 µL	126.272 µL	252.544 µL	1.263 mL	2.525 mL
10 mM	12.627 µL	63.136 µL	126.272 µL	631.361 µL	1.263 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	791.94 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

Adaptive Suppression of MAPT Transcription Maintains Tau Proteostasis in Developing Human Neurons

Authors:

Shin, Mallory R and Firouzshahi, Narges and Liddiard, Gage and Weis, Megan and Simmering, Jacob and Glykys, Joseph and Schultz, Mark and Hefti, Marco M

Lanosterol modulates proteostasis via dissolving cytosolic sequestosomes/aggresome-like induced structures

Authors:

Hu, Li-Dan and Wang, Jing and Chen, Xiang-Jun and Yan, Yong-Bin

Journal:

Biochimica et Biophysica Acta (BBA)-Molecular Cell Research (2019): 118617

Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis.

Authors:

Li, Hao and Chen, Li-Ping and Wang, Tao and Wang, Shao-Gang and Liu, Ji-Hong

Journal:

Asian journal of andrology (2018)

Diabetogenic agent alloxan is a proteasome inhibitor

Authors:

Zhou, Wenjuan and Wei, Lingling and Xiao, Ting and Lai, Chunyou and Peng, Min and Xu, Lingli and Luo, Xiangwei and Deng, Shaoping and Zhang, Fengxue

Journal:

Biochemical and Biophysical Research Communications (2017): 400--406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations

Authors:

Gilda, Jennifer E and Lai, Xianyin and Witzmann, Frank A and Gomes, Aldrin V

Journal:

Molecular & Cellular Proteomics (2016): 1962--1981

References

View all 36 references: Citation Explorer

Enzymatic properties of the 20S proteasome in wheat endosperm and its biochemical characteristics after seed imbibition

Authors:

Shi C, Rui Q, Xu LL.

Journal:

Plant Biol (Stuttg) (2009): 849

Comparative expression analysis and characterization of 20S proteasomes in human intestinal tissues: The proteasome pattern as diagnostic tool for IBD patients

Authors:

Visekruna A, Joeris T, Schmidt N, Lawrenz M, Ritz JP, Buhr HJ, Steinhoff U.

Journal:

Inflamm Bowel Dis (2009): 526

Quinone reductase acts as a redox switch of the 20S yeast proteasome

Authors:

Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey BA, Groll M, Macheroux P.

Journal:

EMBO Rep (2009): 65

Alveolar extracellular 20S proteasome in patients with acute respiratory distress syndrome

Authors:

Sixt SU, Adamzik M, Spyrka D, Saul B, Hakenbeck J, Wohlschlaeger J, Costabel U, Kloss A, Giesebrecht J, Dahlmann B, Peters J.

Journal:

Am J Respir Crit Care Med (2009): 1098

BCL-2 family regulation by the 20S proteasome inhibitor bortezomib

Authors:

Fennell DA, Chacko A, Mutti L.

Journal:

Oncogene (2008): 1189

Physical properties

Molecular weight	791.94
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 24, 2025