Sulfide Green™ AM

Sulfide Green™ AM is a fluorogenic probe for detecting hydrogen sulfide in live cells. It is cell-permeable for use in live cell for imaging sulfide anion or hydrogen sulfide Sulfide Green™ AM contains the hydrophobic acetoxymethyl ester groups, making it cell-permeable. When Sulfide Green™ AM gets in live cells, its AM moieties are cleaved by esterases. The esterase-induced hydrolysis of Sulfide Green™ AM generates a negatively charged fluorogenic product that is well retained in cells. Upon reaction with sulfide or hydrogen sulfide, Sulfide Green generates strong green fluorescence that is proportional to sulfide concentration. The activation of Sulfide Green™ AM has been shown to detect as low as nM sulfide anion in cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21301	10x50 ug	Price

Citations

View all 29 citations: Citation Explorer

Haloalkaliphilic microorganisms assist sulfide removal in a microbial electrolysis cell

Authors:

Ni, G., Harnawan, P., Seidel, L., Ter Heijne, A., Sleutels, T., Buisman, C. J. N., Dopson, M.

Journal:

J Hazard Mater (2019): 197-204

Pharmacological levels of hydrogen sulfide inhibit oxidative cell injury through regulating the redox state of thioredoxin

Authors:

Mao, Z., Huang, Y., Zhang, Z., Yang, X., Zhang, X., Huang, Y., Sawada, N., Mitsui, T., Takeda, M., Yao, J.

Journal:

Free Radic Biol Med (2019): 190-199

Sulfide-mediated azo dye degradation and microbial community analysis in a single-chamber air cathode microbial fuel cell

Authors:

Dai, Q., Zhang, S., Liu, H., Huang, J., Li, L.

Journal:

Bioelectrochemistry (2019): 107349

Corrigendum to "Synergistic growth inhibition of mouse skin tumors by pomegranate fruit extract and diallyl sulfide: Evidence for inhibition of activated MAPKs/NF-kappaB and reduced cell proliferation" [Food Chem. Toxicol. 49 (2011) 1511-20]

Authors:

George, J., Singh, M., Srivastava, A. K., Bhui, K., Shukla, Y.

Journal:

Food Chem Toxicol (2019): 110784

Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria

Authors:

Sahebekhtiari, N., Fern and ez-Guerra, P., Nochi, Z., Carlsen, J., Bross, P., Palmfeldt, J.

Journal:

Biochim Biophys Acta Mol Basis Dis (2019): 126-135

Physical properties

Molecular weight	685.56
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	N/A

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Page updated on September 23, 2025