Sulfide Green™ AM

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	685.56
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

CAS

N/A

Molecular weight

685.56

Extinction coefficient (cm ^-1 M ^-1)

80000

Excitation (nm)

500

Emission (nm)

522

Sulfide Green™ AM is a fluorogenic probe for detecting hydrogen sulfide in live cells. It is cell-permeable for use in live cell for imaging sulfide anion or hydrogen sulfide Sulfide Green™ AM contains the hydrophobic acetoxymethyl ester groups, making it cell-permeable. When Sulfide Green™ AM gets in live cells, its AM moieties are cleaved by esterases. The esterase-induced hydrolysis of Sulfide Green™ AM generates a negatively charged fluorogenic product that is well retained in cells. Upon reaction with sulfide or hydrogen sulfide, Sulfide Green generates strong green fluorescence that is proportional to sulfide concentration. The activation of Sulfide Green™ AM has been shown to detect as low as nM sulfide anion in cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Sulfide Green™ AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	145.866 µL	729.331 µL	1.459 mL	7.293 mL	14.587 mL
5 mM	29.173 µL	145.866 µL	291.732 µL	1.459 mL	2.917 mL
10 mM	14.587 µL	72.933 µL	145.866 µL	729.331 µL	1.459 mL

Molarity calculator

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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Images

Figure 1. Na2S dose response was measured with Sulfide Green™ AM. Na2S standards serially diluted in 20mM HEPES buffer were added to each well to reach the final concentration of 10 µM. The assay was incubated at room temperature for 60 min, and then measure Ex/Em= 490/530nm, cutoff=515nm.

Citations

View all 29 citations: Citation Explorer

Haloalkaliphilic microorganisms assist sulfide removal in a microbial electrolysis cell
Authors: Ni, G., Harnawan, P., Seidel, L., Ter Heijne, A., Sleutels, T., Buisman, C. J. N., Dopson, M.
Journal: J Hazard Mater (2019): 197-204

Pharmacological levels of hydrogen sulfide inhibit oxidative cell injury through regulating the redox state of thioredoxin
Authors: Mao, Z., Huang, Y., Zhang, Z., Yang, X., Zhang, X., Huang, Y., Sawada, N., Mitsui, T., Takeda, M., Yao, J.
Journal: Free Radic Biol Med (2019): 190-199

Sulfide-mediated azo dye degradation and microbial community analysis in a single-chamber air cathode microbial fuel cell
Authors: Dai, Q., Zhang, S., Liu, H., Huang, J., Li, L.
Journal: Bioelectrochemistry (2019): 107349

Corrigendum to "Synergistic growth inhibition of mouse skin tumors by pomegranate fruit extract and diallyl sulfide: Evidence for inhibition of activated MAPKs/NF-kappaB and reduced cell proliferation" [Food Chem. Toxicol. 49 (2011) 1511-20]
Authors: George, J., Singh, M., Srivastava, A. K., Bhui, K., Shukla, Y.
Journal: Food Chem Toxicol (2019): 110784

Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria
Authors: Sahebekhtiari, N., Fern and ez-Guerra, P., Nochi, Z., Carlsen, J., Bross, P., Palmfeldt, J.
Journal: Biochim Biophys Acta Mol Basis Dis (2019): 126-135

Hydrogen sulfide enhances the effectiveness of mesenchymal stem cell therapy in rats with heart failure: In vitro preconditioning versus in vivo co-delivery
Authors: Abdelmonem, M., Shahin, N. N., Rashed, L. A., Amin, H. A. A., Shamaa, A. A., Shaheen, A. A.
Journal: Biomed Pharmacother (2019): 108584

Hydrogen sulfide donor GYY4137 suppresses proliferation of human colorectal cancer Caco-2 cells by inducing both cell cycle arrest and cell death
Authors: Sakuma, S., Minamino, S., Takase, M., Ishiyama, Y., Hosokura, H., Kohda, T., Ikeda, Y., Fujimoto, Y.
Journal: Heliyon (2019): e02244

Simultaneous sulfide removal, nitrogen removal and electricity generation in a coupled microbial fuel cell system
Authors: Chen, Z., Zhang, S., Zhong, L.
Journal: Bioresour Technol (2019): 121888

Facile, rapid one-pot synthesis of multifunctional gold nanoclusters for cell imaging, hydrogen sulfide detection and pH sensing
Authors: Gao, P., Li, M., Zhang, Y., Dong, C., Zhang, G., Shi, L., Li, G., Yuan, M., Shuang, S.
Journal: Talanta (2019): 1-11

Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation
Authors: Wang, P., Chen, F., Wang, W., Zhang, X. D.
Journal: Mediators Inflamm (2019): 8908960