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Sulfide Green™ AM
Sulfide Green™ AM is a fluorogenic probe for detecting hydrogen sulfide in live cells. It is cell-permeable for use in live cell for imaging sulfide anion or hydrogen sulfide Sulfide Green™ AM contains the hydrophobic acetoxymethyl ester groups, making it cell-permeable. When Sulfide Green™ AM gets in live cells, its AM moieties are cleaved by esterases. The esterase-induced hydrolysis of Sulfide Green™ AM generates a negatively charged fluorogenic product that is well retained in cells. Upon reaction with sulfide or hydrogen sulfide, Sulfide Green generates strong green fluorescence that is proportional to sulfide concentration. The activation of Sulfide Green™ AM has been shown to detect as low as nM sulfide anion in cells.
Na2S dose response was measured with Sulfide Green™ AM. Na2S standards serially diluted in 20mM HEPES buffer were added to each well to reach the final concentration of 10 µM. The assay was incubated at room temperature for 60 min, and then measure Ex/Em= 490/530nm, cutoff=515nm.
Na2S dose response was measured with Sulfide Green™ AM. Na2S standards serially diluted in 20mM HEPES buffer were added to each well to reach the final concentration of 10 µM. The assay was incubated at room temperature for 60 min, and then measure Ex/Em= 490/530nm, cutoff=515nm.
CatalogSize
Price
Quantity
2130110x50 ug
Price
 
Physical properties

Molecular weight685.56
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
80000
Excitation (nm)500
Emission (nm)522
Storage, safety and handling

Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
CASN/A
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Page updated on September 23, 2025