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Sulfo-Cyanine 5 NHS ester
A variety of cyanine dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5® dyes are one type of the most common red fluorophores. These versatile fluorophores can tolerate a pH range of 3-10 for use in a variety of applications at biologically relevant pHs. The dyes are also DMSO tolerant and photostable to enable transfer from storage to assay without loss of performance. The aqueous solubility eliminates the need for organic solvents in the assay buffers. Sulfo-Cyanine 5 is an alternative dye primarily used for labeling peptides and oligos in replacing Cy5 that was originally developed by GE Healthcare (Cy5® is the trademark of GE Healthcare). NHS ester dyes are recommended for labeling amine groups and maleimide dyes are recommended for labeling thiol groups. This Cy5® NHS ester readily reacts with amino groups.
Chemical structure for Sulfo-Cyanine 5 NHS ester
Chemical structure for Sulfo-Cyanine 5 NHS ester
CatalogSize
Price
Quantity
26810 mg
Price
26950 mg
Price
 
Physical properties

Molecular weight761.84
SolventDMSO
Spectral properties

Correction factor (260 nm)0.02
Correction factor (280 nm)0.03
Correction factor (482 nm)0.009
Correction factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)651
Emission (nm)670
Quantum yield
0.27
1
0.4
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025