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Sulforhodamine 101, sodium salt

Chemical structure for Sulforhodamine 101, sodium salt
Chemical structure for Sulforhodamine 101, sodium salt
Ordering information
Price ()
Catalog Number71
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight628.69
SolventWater
Spectral properties
Absorbance (nm)573
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.21
Extinction coefficient (cm -1 M -1)139000
Excitation (nm)578
Emission (nm)593
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (56)

OverviewpdfSDSpdfProtocol


CAS
465544-24-5
Molecular weight
628.69
Absorbance (nm)
573
Correction Factor (260 nm)
0.24
Correction Factor (280 nm)
0.21
Extinction coefficient (cm -1 M -1)
139000
Excitation (nm)
578
Emission (nm)
593
Sulforhodamine 101 is a fluorescence reference standard for measuring fluorescence quantum yield. It is also the key precursor used for preparing rhodamine 101 sulfonyl chloride (also called Texas Red®, a trademark of Molecular Probes). This sulforhodamine 101 sodium salt is more water-soluble than the neutral sulforhodamine 101.

Platform


Fluorescence microplate reader

Excitation586 nm
Emission605 nm
Cutoff590 nm
Recommended plateSolid black
Instrument specification(s)TRITC filterset

Example protocol


AT A GLANCE

Important

Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Sulforhodamine 101 stock solution (1mM):
Add 1.591 µL DMSO or water to 1 mg Sulforhodamine 101 to make 1 mM stock solution.  Note: Add appropriate amount of DMSO or water to make appropriate concentration of stock solution.

PREPARATION OF WORKING SOLUTION

1. Sulforhodamine 101 working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7-8. Note: Make appropriate concentration of working solution as per needed.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine 101, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.061 µL795.305 µL1.591 mL7.953 mL15.906 mL
5 mM31.812 µL159.061 µL318.122 µL1.591 mL3.181 mL
10 mM15.906 µL79.53 µL159.061 µL795.305 µL1.591 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)573
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.21
Extinction coefficient (cm -1 M -1)139000
Excitation (nm)578
Emission (nm)593

References


View all 4 references: Citation Explorer
Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31
Flow cytometric applications of Sulforhodamine 101 as a fluorescent stain for total cellular protein
Authors: Engelhard HH., undefined
Journal: Biotech Histochem (1997): 1
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482