Sulforhodamine 101, sodium salt

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Physical properties

Molecular weight	628.69
Solvent	Water

Spectral properties

Absorbance (nm)	573
Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.21
Extinction coefficient (cm ^-1 M ^-1)	139000
Excitation (nm)	578
Emission (nm)	593

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Sulforhodamine 101 sodium salt *CAS 465544-24-5*

Overview SDS Protocol

CAS

465544-24-5

Molecular weight

628.69

Absorbance (nm)

573

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.21

Extinction coefficient (cm ^-1 M ^-1)

139000

Excitation (nm)

578

Emission (nm)

593

Sulforhodamine 101 is a fluorescence reference standard for measuring fluorescence quantum yield. It is also the key precursor used for preparing rhodamine 101 sulfonyl chloride (also called Texas Red®, a trademark of Molecular Probes). This sulforhodamine 101 sodium salt is more water-soluble than the neutral sulforhodamine 101.

Platform

Fluorescence microplate reader

Excitation	586 nm
Emission	605 nm
Cutoff	590 nm
Recommended plate	Solid black
Instrument specification(s)	TRITC filterset

Example protocol

AT A GLANCE

Important

Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Sulforhodamine 101 stock solution (1mM):
Add 1.591 µL DMSO or water to 1 mg Sulforhodamine 101 to make 1 mM stock solution. Note: Add appropriate amount of DMSO or water to make appropriate concentration of stock solution.

PREPARATION OF WORKING SOLUTION

1. Sulforhodamine 101 working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7-8. Note: Make appropriate concentration of working solution as per needed.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine 101, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.061 µL	795.305 µL	1.591 mL	7.953 mL	15.906 mL
5 mM	31.812 µL	159.061 µL	318.122 µL	1.591 mL	3.181 mL
10 mM	15.906 µL	79.53 µL	159.061 µL	795.305 µL	1.591 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	573
Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.21
Extinction coefficient (cm ^-1 M ^-1)	139000
Excitation (nm)	578
Emission (nm)	593

Images

Figure 1. Chemical structure for Sulforhodamine 101, sodium salt

References

View all 4 references: Citation Explorer

Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633

Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31

Flow cytometric applications of Sulforhodamine 101 as a fluorescent stain for total cellular protein
Authors: Engelhard HH., undefined
Journal: Biotech Histochem (1997): 1

Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482