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Sulforhodamine 101, sodium salt

Sulforhodamine 101 is a fluorescence reference standard for measuring fluorescence quantum yield. It is also the key precursor used for preparing rhodamine 101 sulfonyl chloride (also called Texas Red®, a trademark of Molecular Probes). This sulforhodamine 101 sodium salt is more water-soluble than the neutral sulforhodamine 101.

Example protocol

AT A GLANCE

Important

Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Sulforhodamine 101 stock solution (1mM):
Add 1.591 µL DMSO or water to 1 mg Sulforhodamine 101 to make 1 mM stock solution.  Note: Add appropriate amount of DMSO or water to make appropriate concentration of stock solution.

PREPARATION OF WORKING SOLUTION

1. Sulforhodamine 101 working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7-8. Note: Make appropriate concentration of working solution as per needed.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine 101, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.061 µL795.305 µL1.591 mL7.953 mL15.906 mL
5 mM31.812 µL159.061 µL318.122 µL1.591 mL3.181 mL
10 mM15.906 µL79.53 µL159.061 µL795.305 µL1.591 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

References

View all 4 references: Citation Explorer
Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31
Flow cytometric applications of Sulforhodamine 101 as a fluorescent stain for total cellular protein
Authors: Engelhard HH., undefined
Journal: Biotech Histochem (1997): 1
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482
Page updated on December 6, 2024

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Physical properties

Molecular weight

628.69

Solvent

Water

Spectral properties

Absorbance (nm)

573

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.21

Extinction coefficient (cm -1 M -1)

139000

Excitation (nm)

578

Emission (nm)

593

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

465544-24-5

Platform

Fluorescence microplate reader

Excitation586 nm
Emission605 nm
Cutoff590 nm
Recommended plateSolid black
Instrument specification(s)TRITC filterset