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Sulforhodamine 101 sodium salt *CAS 465544-24-5*

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Physical properties
Molecular weight628.69
SolventWater
Spectral properties
Absorbance (nm)573
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.21
Extinction coefficient (cm -1 M -1)139000
Excitation (nm)578
Emission (nm)593
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (46)

OverviewpdfSDSpdfProtocol


CAS
465544-24-5
Molecular weight
628.69
Absorbance (nm)
573
Correction Factor (260 nm)
0.24
Correction Factor (280 nm)
0.21
Extinction coefficient (cm -1 M -1)
139000
Excitation (nm)
578
Emission (nm)
593
Sulforhodamine 101 is a fluorescence reference standard for measuring fluorescence quantum yield. It is also the key precursor used for preparing rhodamine 101 sulfonyl chloride (also called Texas Red®, a trademark of Molecular Probes).

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine 101 sodium salt *CAS 465544-24-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.061 µL795.305 µL1.591 mL7.953 mL15.906 mL
5 mM31.812 µL159.061 µL318.122 µL1.591 mL3.181 mL
10 mM15.906 µL79.53 µL159.061 µL795.305 µL1.591 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)573
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.21
Extinction coefficient (cm -1 M -1)139000
Excitation (nm)578
Emission (nm)593

Images


References


View all 4 references: Citation Explorer
Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31
Flow cytometric applications of Sulforhodamine 101 as a fluorescent stain for total cellular protein
Authors: Engelhard HH., undefined
Journal: Biotech Histochem (1997): 1
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482