Sulforhodamine 101 sulfonyl chloride [Texas Red®] CAS 82354-19-6

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	625.16
Solvent	DMF

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

82354-19-6

Molecular weight

625.16

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.97¹, 0.93²

Sulforhodamine 101 acid chloride (also called Texas Red®) is the most popular labeling reagent of sulfonyl chloride. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.959 µL	799.795 µL	1.6 mL	7.998 mL	15.996 mL
5 mM	31.992 µL	159.959 µL	319.918 µL	1.6 mL	3.199 mL
10 mM	15.996 µL	79.98 µL	159.959 µL	799.795 µL	1.6 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Images

Figure 1. Chemical structure for Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*

Citations

View all 1 citations: Citation Explorer

Functional identification of serine hydroxymethyltransferase as a key gene involved in lysostaphin resistance and virulence potential of Staphylococcus aureus strains
Authors: Batool, Nayab and Ko, Kwan Soo and Chaurasia, Akhilesh Kumar and Kim, Kyeong Kyu
Journal: International journal of molecular sciences (2020): 9135

References

View all 63 references: Citation Explorer

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682

A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133

Bead-based cellular analysis, sorting and multiplexing
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341

Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363

Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494

Budding dynamics of multicomponent tubular vesicles
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996

Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453

Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
Authors: Basu S, Wolgemuth CW, Campagnola PJ.
Journal: Biomacromolecules (2004): 2347

Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
Authors: Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal: Shock (2003): 582