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Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
Sulforhodamine 101 acid chloride (also called Texas Red®) is the most popular labeling reagent of sulfonyl chloride. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.
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Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 159.959 µL | 799.795 µL | 1.6 mL | 7.998 mL | 15.996 mL |
| 5 mM | 31.992 µL | 159.959 µL | 319.918 µL | 1.6 mL | 3.199 mL |
| 10 mM | 15.996 µL | 79.98 µL | 159.959 µL | 799.795 µL | 1.6 mL |
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Citations
View all 1 citations: Citation Explorer
Functional identification of serine hydroxymethyltransferase as a key gene involved in lysostaphin resistance and virulence potential of Staphylococcus aureus strains
Authors: Batool, Nayab and Ko, Kwan Soo and Chaurasia, Akhilesh Kumar and Kim, Kyeong Kyu
Journal: International journal of molecular sciences (2020): 9135
Authors: Batool, Nayab and Ko, Kwan Soo and Chaurasia, Akhilesh Kumar and Kim, Kyeong Kyu
Journal: International journal of molecular sciences (2020): 9135
References
View all 63 references: Citation Explorer
Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682
A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133
Bead-based cellular analysis, sorting and multiplexing
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341
Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363
Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
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