Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
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Additional ordering information
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Physical properties
Molecular weight | 625.16 |
Solvent | DMF |
Spectral properties
Correction Factor (260 nm) | 0.23 |
Correction Factor (280 nm) | 0.36 |
Correction Factor (565 nm) | 0.4472 |
Extinction coefficient (cm -1 M -1) | 1160001 |
Excitation (nm) | 586 |
Emission (nm) | 603 |
Quantum yield | 0.971, 0.932 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
CAS 82354-19-6 | Molecular weight 625.16 | Correction Factor (260 nm) 0.23 | Correction Factor (280 nm) 0.36 | Correction Factor (565 nm) 0.4472 | Extinction coefficient (cm -1 M -1) 1160001 | Excitation (nm) 586 | Emission (nm) 603 | Quantum yield 0.971, 0.932 |
Sulforhodamine 101 acid chloride (also called Texas Red®) is the most popular labeling reagent of sulfonyl chloride. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 159.959 µL | 799.795 µL | 1.6 mL | 7.998 mL | 15.996 mL |
5 mM | 31.992 µL | 159.959 µL | 319.918 µL | 1.6 mL | 3.199 mL |
10 mM | 15.996 µL | 79.98 µL | 159.959 µL | 799.795 µL | 1.6 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.23 |
Correction Factor (280 nm) | 0.36 |
Correction Factor (565 nm) | 0.4472 |
Extinction coefficient (cm -1 M -1) | 1160001 |
Excitation (nm) | 586 |
Emission (nm) | 603 |
Quantum yield | 0.971, 0.932 |
Images
Citations
View all 1 citations: Citation Explorer
Functional identification of serine hydroxymethyltransferase as a key gene involved in lysostaphin resistance and virulence potential of Staphylococcus aureus strains
Authors: Batool, Nayab and Ko, Kwan Soo and Chaurasia, Akhilesh Kumar and Kim, Kyeong Kyu
Journal: International journal of molecular sciences (2020): 9135
Authors: Batool, Nayab and Ko, Kwan Soo and Chaurasia, Akhilesh Kumar and Kim, Kyeong Kyu
Journal: International journal of molecular sciences (2020): 9135
References
View all 63 references: Citation Explorer
Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682
A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133
Bead-based cellular analysis, sorting and multiplexing
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341
Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363
Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
Budding dynamics of multicomponent tubular vesicles
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996
Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453
Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455
Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
Authors: Basu S, Wolgemuth CW, Campagnola PJ.
Journal: Biomacromolecules (2004): 2347
Authors: Basu S, Wolgemuth CW, Campagnola PJ.
Journal: Biomacromolecules (2004): 2347
Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
Authors: Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal: Shock (2003): 582
Authors: Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal: Shock (2003): 582
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)