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Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*

Chemical structure for Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
Chemical structure for Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
Ordering information
Price ()
Catalog Number480
Unit Size
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Additional ordering information
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Fax1-408-733-1304
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight625.16
SolventDMF
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Sulforhodamine 101 *CAS 60311-02-6*
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Show More (58)

OverviewpdfSDSpdfProtocol


CAS
82354-19-6
Molecular weight
625.16
Correction Factor (260 nm)
0.23
Correction Factor (280 nm)
0.36
Correction Factor (565 nm)
0.4472
Extinction coefficient (cm -1 M -1)
1160001
Excitation (nm)
586
Emission (nm)
603
Quantum yield
0.931, 0.352
Sulforhodamine 101 acid chloride (also called Texas Red®) is the most popular labeling reagent of sulfonyl chloride. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.

Calculators


Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.959 µL799.795 µL1.6 mL7.998 mL15.996 mL
5 mM31.992 µL159.959 µL319.918 µL1.6 mL3.199 mL
10 mM15.996 µL79.98 µL159.959 µL799.795 µL1.6 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352

Citations


View all 1 citations: Citation Explorer
Functional identification of serine hydroxymethyltransferase as a key gene involved in lysostaphin resistance and virulence potential of Staphylococcus aureus strains
Authors: Batool, Nayab and Ko, Kwan Soo and Chaurasia, Akhilesh Kumar and Kim, Kyeong Kyu
Journal: International journal of molecular sciences (2020): 9135

References


View all 63 references: Citation Explorer
Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
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A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
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Journal: Eur J Pharm Sci (2005): 133
Bead-based cellular analysis, sorting and multiplexing
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Journal: Chembiochem (2005): 1341
Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363
Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
Budding dynamics of multicomponent tubular vesicles
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996
Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453
Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455
Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
Authors: Basu S, Wolgemuth CW, Campagnola PJ.
Journal: Biomacromolecules (2004): 2347
Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
Authors: Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal: Shock (2003): 582