SunRed™ Phosphate

Phosphatase-catalyzed hydrolysis of Sun Red phosphate (SRP) yields the Sun Red fluorophore that can be excited with the 633 nm laser with emission of ~660 nm. Although Sun Red is readily excited at 633 nm with red emission of ~660 nm, SRP has very minimal absorption at 633 nm without red emission, making SRP one of the most sensitive NIR phosphatase sensors. Please do not use DMSO to make stock solution since it significantly increases assay background.

Alkaline phosphatase dose response was measured with the Amplite® Fluorimetric Alkaline Phosphatase Assay Kit in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11629	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of SunRed™ Phosphate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	236.855 µL	1.184 mL	2.369 mL	11.843 mL	23.685 mL
5 mM	47.371 µL	236.855 µL	473.709 µL	2.369 mL	4.737 mL
10 mM	23.685 µL	118.427 µL	236.855 µL	1.184 mL	2.369 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	422.2 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

DNA condensation induced by cationic surfactant: a viscosimetry and dynamic light scattering study

Authors:

Marchetti S, Onori G, Cametti C.

Journal:

J Phys Chem B (2005): 3676

Simultaneous trichromatic fluorescence detection of proteins on Western blots using an amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates

Authors:

Martin K, Hart C, Liu J, Leung WY, Patton WF.

Journal:

Proteomics (2003): 1215

Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes

Authors:

Top KP, Hatleberg G, Berggren KN, Ryan D, Kemper C, Haugl and RP, Patton WF.

Journal:

Electrophoresis (2001): 896

Characterization of 9H-(1,3-dichlor-9, 9-dimethylacridin-2-ona-7-yl)-phosphate (DDAO) as substrate of PP-2A in a fluorimetric microplate assay for diarrhetic shellfish toxins (DSP)

Authors:

Leira F, Vieites JM, Vieytes MR, Botana LM.

Journal:

Toxicon (2000): 1833

A column centrifugation method for the reconstitution in liposomes of the mitochondrial F0F1 ATP synthase/ATPase

Authors:

Vazquez-Contreras E, de Gomez-Puyou MT, Dreyfus G.

Journal:

Protein Expr Purif (1996): 55

Physical properties

Molecular weight	422.20
Solvent	Water

Spectral properties

Excitation (nm)	653
Emission (nm)	661

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	620 nm
Emission	660 nm
Cutoff	640 nm
Recommended plate	Solid black

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Page updated on October 8, 2025