SunRed™ SE

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1123.34
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.366
Excitation (nm)	592
Emission (nm)	609

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1123.34

Correction Factor (280 nm)

0.366

Excitation (nm)

592

Emission (nm)

609

Although sulforhodamine 101 acid chloride (also called Texas Red®) is the most popular labeling reagent of sulfonyl chloride, it is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Texas Red (TR) reacts with both aliphatic amines and aromatic amines indiscriminatingly. In addition, the labeling efficiency of Texas Red is extremely low compared to dye succinimidyl esters. SunRed™ SE is a succinimidyl ester. It is an excellent replacement for Texas Red. SunRed reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Texas Red, SunRed has much higher labeling efficiency, and more importantly the resulted conjugate is more fluorescent and water soluble than the corresponding Texas Red conjugate. The conjugates of SunRed have the identical excitation and emission wavelengths to those of Texas Red. Our in-house studies indicated that SunRed is more stable than Texas Red under the same labeling conditions. It is extremely useful for labeling hydrophobic peptides, giving the fluorescent conjugates that are much easier to be purified and more water soluble to use.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of SunRed™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.02 µL	445.101 µL	890.202 µL	4.451 mL	8.902 mL
5 mM	17.804 µL	89.02 µL	178.04 µL	890.202 µL	1.78 mL
10 mM	8.902 µL	44.51 µL	89.02 µL	445.101 µL	890.202 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.366
Excitation (nm)	592
Emission (nm)	609

Product Family

Name	Excitation (nm)	Emission (nm)
SunRed™ Acetate	653	661
SunRed™ Phosphate	653	661
DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] CAS 139346-57-9	411	472
CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] Mixed isomers	505	526

Images

Figure 1. Texas Red® and SunRed™ SE were respectively mixed with Gly-gly-Ser-Ser-Arg-Trp in 1:1 molar ratio at pH=9.0 under the same conditions.The resulted conjugates were purified by HPLC using a reverse phase C18 column.

References

View all 63 references: Citation Explorer

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682

A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133

Bead-based cellular analysis, sorting and multiplexing
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341

Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363

Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494

Budding dynamics of multicomponent tubular vesicles
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996

Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453

Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
Authors: Basu S, Wolgemuth CW, Campagnola PJ.
Journal: Biomacromolecules (2004): 2347

Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
Authors: Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal: Shock (2003): 582