logo
AAT Bioquest

SYBR Dye qPCR Calibration Solution *10,000X*

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as SYBR, will depend on the device used. The AAT Bioquest SYBR dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.

Example protocol

AT A GLANCE

Materials Required but Not Supplied
  1. DNA Stock Solution (1 mg/mL)

SAMPLE EXPERIMENTAL PROTOCOL

This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.

Prepare a SYBR calibration plate:
  1. Prepare a SYBR Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X SYBR Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.

    Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  2. Add 100 µL of DNA stock solution (1 mg/mL) in 900 µL of SYBR Dye qpCR calibration solution and mix well.

    Note: The DNA stock solution is not supplied, but required for calibration.

  3. Add 20 µL of the SYBR Dye qPCR Calibration working solution to all wells of a reaction plate.

    Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  4. Seal the plate with an optical adhesive cover.

  5. Centrifuge the plate for 2 minutes at < 1500 rpm.

  6. Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.

  7. For complete calibration instructions, refer to the maintenance guide for your instrument.

Spectrum

Page updated on November 9, 2024

Ordering information

Price
Unit size
Catalog Number67035
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Solvent

Water

Spectral properties

Excitation (nm)

498

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Platform

qPCR

Recommended platePCR Microplate
Instrument specification(s)SYBR Filter
ABI 7500 FAST system SYBR dye spectrum.
ABI 7500 FAST system SYBR dye spectrum.
ABI 7500 FAST system SYBR dye spectrum.