TAMRA-cGMP PDE V substrate *Red Fluorescence*

TAMRA-cGMP PDE V substrate

Red Fluorescence

This red cGMP derivative is a specific substrate for phosphodiesterase (PDE) V. It can be used for assaying PDE V activities or screening PDE V inhibitors in combination with anti-cGMP antibody in a FRET readout or FP format. PDE is a group of enzymes that degrade the second messenger molecules: cyclic nucleotides cAMP and cGMP. They regulate the localization, duration, and amplitude of cyclic nucleotide signaling within subcellular domains. PDEs are therefore important regulators of signal transduction mediated by these second messenger molecules. PDE enzymes are often targets for pharmacological inhibition due to their unique tissue distribution, structural and functional properties. Inhibitors of PDE can prolong or enhance the effects of physiological processes mediated by cAMP or cGMP by inhibition of their degradation by PDE. PDE inhibitors have been identified as new potential therapeutics in areas such as pulmonary arterial hypertension, coronary heart disease, dementia, depression and schizophrenia. For example, Sildenafil (Viagra) is an inhibitor of cGMP-specific PDE V, which enhances the vasodilatory effects of cGMP in the corpus cavernosum, and is used to treat erectile dysfunction.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13605	0.5 umol	Price

References

View all 34 references: Citation Explorer

DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells

Authors:

Bader S, Kortholt A, Snippe H, Van Haastert PJ.

Journal:

J Biol Chem (2006): 20018

Expression of cAMP and cGMP-phosphodiesterase isoenzymes 3, 4, and 5 in the human clitoris: immunohistochemical and molecular biology study

Authors:

Oelke M, Hedlund P, Albrecht K, Ellinghaus P, Stief CG, Jonas U, Andersson KE, Uckert S.

Journal:

Urology (2006): 1111

Immunohistochemical distribution of cAMP- and cGMP-Phosphodiesterase (PDE) isoenzymes in the human prostate

Authors:

Sampaio FJ., undefined

Journal:

Int Braz J Urol (2006): 368

Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5

Authors:

Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, Houslay MD.

Journal:

Biochem J (2006): 23

cAMP phosphodiesterase-4A1 (PDE4A1) has provided the paradigm for the intracellular targeting of phosphodiesterases, a process that underpins compartmentalized cAMP signalling

Authors:

Huston E, Houslay TM, Baillie GS, Houslay MD.

Journal:

Biochem Soc Trans (2006): 504

Physical properties

Molecular weight	~1000
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 13, 2025