Name: TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
Brand: AAT Bioquest
SKU: 17292
Availability: InStock

TAQuest™ FAST qPCR Master Mix for TaqMan Probes

High ROX

TAQuest™ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. The master mix is compatible with FAST conditions, thus delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The master mix provides all of the essential components including our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer, except the template, primers and probes. TAQuest™ FAST qPCR Master Mix for TaqMan Probes has been designed to be used for duplex reactions using internal positive controls with superior performance. The master mix ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. This master mix contains a high amount of ROX reference dye.

General packaging for catalogs 17292 and 17293. Actual shipped packaging may vary.

Protocol

COA

Catalog	Size	Price	Quantity
17292	1 mL	Price
17293	5 mL	Price

References

View all 9 references: Citation Explorer

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.

Authors:

Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O'Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa

Journal:

PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.

Authors:

Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin

Journal:

Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.

Authors:

Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R

Journal:

Journal of medical virology (2016): 115-9

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.

Authors:

Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix

Journal:

BioTechniques (2014): 151-5

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.

Authors:

Schrell, Adrian M and Roper, Michael G

Journal:

The Analyst (2014): 2695-701

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

qPCR
Instrument specification(s)	Filter based on probes

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Page updated on October 16, 2025