TAQuest™ FAST qPCR Master Mix for TaqMan Probes High ROX

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

Overview SDS Protocol

TAQuest™ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. The master mix is compatible with FAST conditions, thus delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The master mix provides all of the essential components including our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer, except the template, primers and probes. TAQuest™ FAST qPCR Master Mix for TaqMan Probes has been designed to be used for duplex reactions using internal positive controls with superior performance. The master mix ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. This master mix contains a high amount of ROX reference dye.

Platform

qPCR

Instrument specification(s)

Filter based on probes

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.
Note Thaw the TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* at room temperature. Vortex qPCR Master Mix thoroughly before use.

Prepare one of the following reaction mixes as indicated in Table 1.
Carefully mix the reagents with a gentle vortex followed by a brief centrifuge.
Set up the plate in the qPCR instrument and run as indicated in Table 2.

Table 1.Reagents composition per well for each reaction

Components	Volume (25 µL/reaction)	Volume (50 µL/reaction)	Final Conc.
TAQuest™ FAST qPCR Master Mix for TaqMan Probes High ROX	12.5 µL	25 µL	1X
Upstream primer, 10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
Downstream primer, 10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
TaqMan Probes, 10 µM	0.25-0.625 µL	0.5-1.25 µL	100-250 nM
DNA template	1-5 µL	1-5 µL	Optimized conc.
Nuclease-Free Water to	25 µL	50 µL

Table 2.Thermal cycling parameters

Parameter	Polymerase Activation	PCR (30-40 cycles)
	Hold	Denature	Anneal/Extend
Temperature	95 °C	95 °C	60 °C
Time (m:ss)	0:10	0:20	0:30

References

View all 9 references: Citation Explorer

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O'Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia : 62-70

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia : 57-61