TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green

Low ROX

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The mix includes an optimized buffer containing dNTPs and our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme, an enzyme designed to allow instant hot start which minimizes non-specific product formation thus allowing room temperature reaction setup. Only template and target primers are required to run the desired PCR reactions. TAQuest™ FAST qPCR Master Mix with Helixyte™ Green ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix contains a low amount of ROX reference dye.

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*.

Protocol

COA

Catalog	Size	Price	Quantity
17278	1 mL	Price
17279	5 mL	Price

References

View all 10 references: Citation Explorer

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).

Authors:

Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D'Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura

Journal:

BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).

Authors:

Kang, Tae Sun

Journal:

Food chemistry (2019): 1-8

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.

Authors:

Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun

Journal:

Food chemistry (2018): 112-119

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.

Authors:

Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo

Journal:

Journal of microbiological methods (2018): 12-17

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).

Authors:

Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J

Journal:

Veterinary sciences (2018)

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

qPCR
Instrument specification(s)	SYBR Green filter

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Page updated on October 12, 2025