Name: TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
Brand: AAT Bioquest
SKU: 17272
Availability: InStock

TAQuest™ qPCR Master Mix with Helixyte™ Green

Low ROX

TAQuest™ qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix includes our proprietary TAQuest™ Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer. You only need to add template and target primers to run the desired PCR reactions. The Hot Start Taq DNA polymerase allows you to set up a PCR reaction at room temperature, thus minimizing non-specific product formation. In combination with an optimized buffer, the enzyme ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix contains a low amount of ROX reference dye.

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*.

Protocol

COA

Catalog	Size	Price	Quantity
17272	1 mL	Price
17273	5 mL	Price

References

View all 37 references: Citation Explorer

Development of cycling probe based real-time PCR methodology for influenza A viruses possessing the PA/I38T amino acid substitution associated with reduced baloxavir susceptibility.

Authors:

Osada, Hidekazu and Chon, Irina and Phyu, Wint Wint and Wagatsuma, Keita and Nagata, Nobuo and Kawashima, Takashi and Sato, Isamu and Saito, Tadashi and Kodo, Naoki and Masaki, Hironori and Asoh, Norichika and Tsuchihashi, Yoshiko and Shirahige, Yutaka and Ono, Yasuhiko and Shimada, Yasushi and Hamabata, Hirotsune and Saito, Kousuke and Saito, Reiko

Journal:

Antiviral research (2021): 105036

Identification of a MarR subfamily that regulates arsenic resistance genes.

Authors:

Yu, Yanshuang and Chen, Jichen and Li, Yuanping and Liang, Jinxuan and Xie, Zhenchen and Feng, Renwei and Alwathnani, Hend A and Rosen, Barry P and Grove, Anne and Chen, Jian and Rensing, Christopher

Journal:

Applied and environmental microbiology (2021): AEM0158821

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.

Authors:

Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O'Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa

Journal:

PloS one (2021): e0256813

Method for detecting hepatitis B virus in blood plasma at low viral load using real-time PCR.

Authors:

Serikova, E N and Semenov, A V and Ostankova, Yu V and Totolian, Areg A

Journal:

Klinicheskaia laboratornaia diagnostika (2021): 59-64

SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.

Authors:

Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A

Journal:

Mymensingh medical journal : MMJ (2020): 596-600

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

qPCR
Instrument specification(s)	SYBR Green filter

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Page updated on October 12, 2025