Name: TCO-amine hydrochloride
Brand: AAT Bioquest
SKU: 4536
Price: 95 USD
Availability: InStock

TCO-amine hydrochloride

TCO-Amine Hydrochloride is a water-soluble click chemistry reagent that enables rapid, biocompatible conjugation reactions with tetrazines under physiological conditions.

Reactive Functional Group: Primary amine allows for efficient covalent coupling to carboxyl-containing molecules (e.g., NHS esters) for bioconjugation
Bioorthogonal Ligand: TCO enables rapid and selective inverse electron-demand Diels–Alder (IEDDA) cycloaddition with tetrazine derivatives
High Reactivity: Supports fast, catalyst-free conjugation under physiological conditions
Enhanced Solubility and Stability: Supplied as a hydrochloride salt to improve aqueous solubility and chemical stability during storage and handling

The chemical structure of TCO-amine hydrochloride.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
4536	5 mg	Price

Overview

TCO-amine hydrochloride is a water-soluble, amine-functionalized trans-cyclooctene (TCO) derivative optimized for bioorthogonal conjugation via the inverse electron-demand Diels–Alder (IEDDA) reaction with tetrazines. The primary amine group enables efficient coupling to electrophilic functional groups such as N-hydroxysuccinimide (NHS) esters or activated carboxylic acids, facilitating covalent attachment to biomolecules including proteins, peptides, and oligonucleotides. The hydrochloride salt form enhances aqueous solubility and improves handling and storage stability. Owing to the rapid kinetics, high specificity, and biocompatibility of the TCO–tetrazine ligation, TCO-amine hydrochloride is particularly well suited for applications requiring fast and selective labeling under physiological conditions. These include live-cell and in vivo imaging, pretargeted therapeutic strategies, and site-specific modification of biomolecules in complex biological systems.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of TCO-amine hydrochloride to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	380.546 µL	1.903 mL	3.805 mL	19.027 mL	38.055 mL
5 mM	76.109 µL	380.546 µL	761.093 µL	3.805 mL	7.611 mL
10 mM	38.055 µL	190.273 µL	380.546 µL	1.903 mL	3.805 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	262.78 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Positron Emission Tomography and Optical Imaging to Monitor Bioorthogonal Diels-Alder Click Chemistry of Trastuzumab with a Porphyrin.

Authors:

Gamelas, Sara R D and Shmuel, Shayla and Simó, Cristina and Vanover, Alex and Tomé, João P C and Tomé, Augusto C and Lourenço, Leandro M O and Pereira, Patrícia M R

Journal:

Bioconjugate chemistry (2025)

Enhanced mRNA delivery using ultrasound-delivered click reactive anchors.

Authors:

Di Ianni, Emilio and Jeon, Jueun and Hu, Huiyu and Quintana, Jeremy M and Lee, Chanseo and Haidar, Edwina Abou and Mohammadi, Sedra and Zargani-Piccardi, Ayrton and Mahamdeh, Mohammed and Hernández, Iván Coto and Ng, Thomas S C and Breyne, Koen and Lee, Hakho and Breakefield, Xandra O and Miller, Miles A

Journal:

bioRxiv : the preprint server for biology (2025)

Easy Access to Bioorthogonal Click-to-Release Reagent Bishydroxy-trans-cyclooctene (C2TCO) and Harnessing of Its Rapid Labeling and Dissecting Feature in Multicycle Imaging.

Authors:

Arun, V and Lee, Minju and Choi, Hongseo and Lee, Sangwoo and Choi, Junwon and Yoo, Tae Hyeon and Kim, Wook and Kim, Eunha

Journal:

Bioconjugate chemistry (2025)

Development of a Novel Prosthetic Click-Linker for Radioiodination of Antibody-Based Radiopharmaceuticals with High Stability and Specificity.

Authors:

Im, Changkeun and Ahn, Jae Hun and Lim, Hwisoo and Kim, Dohyeon and Lee, Yong Jin and Kang, Chi Soo and Kang, Choong Mo

Journal:

Molecular pharmaceutics (2025): 295-303

Synthesis and Characterization of Click Chemical Probes for Single-Cell Resolution Detection of Epichaperomes in Neurodegenerative Disorders.

Authors:

Bay, Sadik and Digwal, Chander S and Rodilla Martín, Ananda M and Sharma, Sahil and Stanisavljevic, Aleksandra and Rodina, Anna and Attaran, Anoosha and Roychowdhury, Tanaya and Parikh, Kamya and Toth, Eugene and Panchal, Palak and Rosiek, Eric and Pasala, Chiranjeevi and Arancio, Ottavio and Fraser, Paul E and Alldred, Melissa J and Prado, Marco A M and Ginsberg, Stephen D and Chiosis, Gabriela

Journal:

Biomedicines (2024)

Physical properties

Molecular weight	262.78
Solvent	DMF

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
CAS	1609736-43-7

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 9, 2025