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Hoechst 33342 is a cell membrane-permeant fluorescent DNA stain with robust live-cell imaging compatibility, ideal for flow cytometry, side population assays, apoptosis detection, and more.
Product description
Hoechst 33342 is a fluorescent DNA stain widely acclaimed for its strong binding affinity to A–T-rich regions in the minor groove of double-stranded DNA. This dye surpasses many nuclear stains due to its additional ethyl substituent, which boosts membrane permeability. As a result, Hoechst 33342 seamlessly crosses the plasma membrane of live cells, making it an unrivaled tool in fields such as cell biology, immunology, neuroscience, and high-throughput drug discovery.
At AAT Bioquest, we are committed to delivering high-purity and easy-to-use Hoechst 33342 solutions, formulated for both live-cell and fixed-cell applications. Built on decades of fluorescence chemistry expertise, our brand guarantees:
- Optimal brightness under UV or near-UV excitation
- Low background noise for clean nuclear segmentation
- High consistency across batches due to rigorous QC
- Proven protocols that stand out in side-by-side comparisons
Why This Matters: Whether you need robust cell cycle analysis, reliable apoptosis detection, or advanced multi-channel imaging, Hoechst 33342 is the go-to nuclear stain—offering you the best in class for both routine staining and cutting-edge research.
More Details
Hoechst 33342 is part of the Hoechst dye family (which includes Hoechst 33258 and Hoechst 34580), originally developed by Hoechst AG. These bis-benzimides exhibit strong affinity for nuclear DNA; however, the unique chemical structure of Hoechst 33342 imparts superior cell permeability (10-fold greater than Hoechst 33258), ensuring efficient entry into live cells without permeabilization.
Since its introduction, Hoechst 33342 has been adopted in numerous research disciplines, from fundamental cell biology and immunology to advanced neuroscience and high-throughput drug discovery. Its compatibility with standard UV or near-UV laser lines (e.g., 355 nm, 405 nm) makes it exceptionally versatile in confocal fluorescence imaging, flow cytometry, super-resolution microscopy, high-content screening, and fluorescence lifetime imaging. Studies have also leveraged its preference for A–T–rich DNA in specialized experiments such as DNA conformational analysis and epigenetic mapping.
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Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
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Common stock solution preparation
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.786 mL | 8.929 mL | 17.857 mL | 89.286 mL | 178.571 mL |
| 5 mM | 357.143 µL | 1.786 mL | 3.571 mL | 17.857 mL | 35.714 mL |
| 10 mM | 178.571 µL | 892.857 µL | 1.786 mL | 8.929 mL | 17.857 mL |
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