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AAT Bioquest

TET-dT Phosphoramidite

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Physical properties
Molecular weight1563.35
SolventMeCN
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
1563.35
TET-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the TET tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-TET phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer fluorescein-dT phosphoramidite, HEX-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.

Calculators


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of TET-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM63.965 µL319.826 µL639.652 µL3.198 mL6.397 mL
5 mM12.793 µL63.965 µL127.93 µL639.652 µL1.279 mL
10 mM6.397 µL31.983 µL63.965 µL319.826 µL639.652 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*521542730000.13
HEX-dT Phosphoramidite533559730000.15
Fluorescein-dT Phosphoramidite4985178000010.35

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References


View all 1 references: Citation Explorer
Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.
Authors: Riml, Christian and Micura, Ronald
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 295-302