TET-dT Phosphoramidite
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1563.35 |
Solvent | MeCN |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Peptide and Oligonucleotide Labeling
Molecular weight 1563.35 |
TET-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the TET tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-TET phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer fluorescein-dT phosphoramidite, HEX-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of TET-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 63.965 µL | 319.826 µL | 639.652 µL | 3.198 mL | 6.397 mL |
5 mM | 12.793 µL | 63.965 µL | 127.93 µL | 639.652 µL | 1.279 mL |
10 mM | 6.397 µL | 31.983 µL | 63.965 µL | 319.826 µL | 639.652 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6* | 521 | 542 | 73000 | 0.13 |
HEX-dT Phosphoramidite | 533 | 559 | 73000 | 0.15 |
Fluorescein-dT Phosphoramidite | 498 | 517 | 800001 | 0.35 |
References
View all 1 references: Citation Explorer
Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.
Authors: Riml, Christian and Micura, Ronald
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 295-302
Authors: Riml, Christian and Micura, Ronald
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 295-302
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