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Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)*

Documents
pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Related catalogs
Fluorescent in situ hybridization (FISH)
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Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
Calcein
AssayWise
Glycerol 3-Phosphate Assays
Nucleic Acid Quantification
Hydroxyl Radical Detection
Thiol Detection
Nucleic Acid Detection, Quantification and Imaging
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TMR-dUTP conjugate can be used as an orange fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis).
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Catalog Number17023
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Physical properties
Molecular weight1115.77
SolventWater
Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
TF1-dUTP *1 mM in TE Buffer (pH 7.5)*42046135000-
TF2-dUTP *1 mM in TE Buffer (pH 7.5)*503525750000.09
TF3-dUTP *1 mM in TE Buffer (pH 7.5)*5545787500010.179
TF4-dUTP *1 mM in TE Buffer (pH 7.5)*578602900000.436
TF5-dUTP *1 mM in TE Buffer (pH 7.5)*6496642500000.027
DEAC-dUTP *1 mM in TE Buffer (pH 7.5)*411472-0.12
References
View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
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