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Texas Red® maleimide *Single Isomer*

Texas Red® maleimide is an excellent thiol-reactive fluorescent reagent for labeling thiol-containing biological molecules (such as peptides, proteins and thiol-modified oligonucleotides). It selectively reacts with thiol groups of peptides, proteins and other biological molecules (such as small thiol-containing organic molecules). It has the spectral properties identical to those of Texas Red conjugates, thus all the instrument settings of the Texas Red conjugates would be applicable to the applications of TR C2 maleimide conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red® maleimide *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM137.206 µL686.031 µL1.372 mL6.86 mL13.721 mL
5 mM27.441 µL137.206 µL274.412 µL1.372 mL2.744 mL
10 mM13.721 µL68.603 µL137.206 µL686.031 µL1.372 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (565 nm)
Texas Red-DHPE *CAS 187099-99-6*58660311600010.971, 0.9320.230.360.4472
Texas Red TCO *Single isomer*58660311600010.971, 0.9320.230.360.4472

References

View all 63 references: Citation Explorer
Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682
A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
Authors: Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: Eur J Pharm Sci (2005): 133
Bead-based cellular analysis, sorting and multiplexing
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341
Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363
Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
Page updated on October 22, 2024

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Physical properties

Molecular weight

728.83

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm -1 M -1)

1160001

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.971, 0.932

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501