logo
AAT Bioquest

Texas Red®-streptavidin conjugate

HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by Texas Red®-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by Texas Red®-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by Texas Red®-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.971, 0.932
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5*
Texas Red® hydrazide *Single Isomer*
Texas Red® cadaverine *Single Isomer*
Texas Red® maleimide *Single Isomer*
Texas Red® azide *Single Isomer*
Texas Red® alkyne *Single Isomer*
Texas Red® goat anti-mouse IgG (H+L)
Texas Red® goat anti-mouse IgG (H+L) *Cross Adsorbed*
Texas Red® goat anti-rabbit IgG (H+L)
Texas Red® goat anti-rabbit IgG (H+L) *Cross Adsorbed*
Texas Red-DHPE *CAS 187099-99-6*
Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5*
Texas Red TCO *Single isomer*
Texas Red-X, acid *CAS 199745-67-0*
RPE-streptavidin conjugate
APC-streptavidin conjugate
PerCP-streptavidin conjugate
RPE-iFluor® 647-streptavidin conjugate
RPE-iFluor® 750-streptavidin conjugate
APC-iFluor® 750-streptavidin conjugate
FITC-streptavidin conjugate
Cy3®-streptavidin conjugate
Cy5®-streptavidin conjugate
Cy7®-streptavidin conjugate
HRP-streptavidin conjugate
AP-streptavidin conjugate [Streptavidin-alkaline phosphatase conjugate]
trFluor™ Eu-streptavidin conjugate
trFluor™ Tb-streptavidin conjugate
mFluor™ Violet 450-streptavidin conjugate
mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
iFluor® 350-streptavidin conjugate
iFluor® 405-streptavidin conjugate
iFluor® 488-streptavidin conjugate
iFluor® 514-streptavidin conjugate
iFluor® 532-streptavidin conjugate
iFluor® 555-streptavidin conjugate
iFluor® 633-streptavidin conjugate
iFluor® 647-streptavidin conjugate
iFluor® 680-streptavidin conjugate
iFluor® 700-streptavidin conjugate
iFluor® 750-streptavidin conjugate
iFluor® 546-streptavidin conjugate
iFluor® 568-streptavidin conjugate
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
RPE-Cy7-streptavidin conjugate
iFluor® 790-streptavidin conjugate
iFluor® 800-streptavidin conjugate
iFluor® 820-streptavidin conjugate
iFluor® 840-streptavidin conjugate
iFluor® 860-streptavidin conjugate
MegaWox™ polyHRP-Streptavidin Conjugate
RPE-Cy5-streptavidin conjugate
mFluor™ UV455-streptavidin conjugate
mFluor™ Violet 545-streptavidin conjugate
mFluor™ Violet 550-streptavidin conjugate
mFluor™ Violet 590-streptavidin conjugate
mFluor™ Violet 610-streptavidin conjugate
mFluor™ UV420-streptavidin conjugate
Show More (56)

OverviewpdfSDSpdfProtocol


Molecular weight
~52000
Correction Factor (260 nm)
0.23
Correction Factor (280 nm)
0.36
Correction Factor (565 nm)
0.4472
Extinction coefficient (cm -1 M -1)
1160001
Excitation (nm)
586
Emission (nm)
603
Quantum yield
0.971, 0.932
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Texas Red-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Texas Red covalently attached (as the fluorescent label). Our Texas Red-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Texas Red-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Texas Red filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.971, 0.932

Images


Citations


View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References


View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517
Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576
Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73