Name: Texas Red-X, ortho succinimidyl ester *Single isomer*
Brand: AAT Bioquest
SKU: 476
Price: 695 USD
Availability: InStock

Texas Red-X, ortho succinimidyl ester

Single isomer

Texas Red®-X Ortho Succinimidyl Ester (Single Isomer) is a bright red fluorescent dye with enhanced conjugation efficiency and reduced steric hindrance, ideal for precise protein labeling applications.

Single Ortho-Isomer Purity: Provides reproducible labeling results compared to mixed isomer preparations
Seven-Atom Spacer Arm: Reduces steric hindrance and quenching for improved conjugate brightness
Enhanced Texas Red Alternative: Superior conjugation efficiency while maintaining spectral properties

The chemical structure of Texas Red-X, ortho succinimidyl ester *single isomer*.

Protocol

SDS

Catalog	Size	Price	Quantity
476	5 mg	Price

Overview

The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of ThermoFisher). Most commercial vendors only offer the mixed isomers of para and ortho positions. AAT Bioquest is the only vendor that offers the single isomer of both para (#474) and ortho isomer (#476), and their mixed isomers (#475). The para (#474) and ortho isomer (#476) have almost the same spectral properties to Texas Red. They can be used to create bright red-fluorescent bioconjugates with excitation/emission maxima ~595/615 nm. Compared to Texas Red®, Texas Red®-X dye contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the succinimidyl ester group. This spacer helps to separate the fluorophore from its point of attachment, potentially reducing the interaction of the fluorophore with the biomolecule to which it is conjugated. Compared to the mixed isomers (#474), this product is the single 2-isomer, giving more reproducible results.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-X, ortho succinimidyl ester *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.408 µL	612.04 µL	1.224 mL	6.12 mL	12.241 mL
5 mM	24.482 µL	122.408 µL	244.816 µL	1.224 mL	2.448 mL
10 mM	12.241 µL	61.204 µL	122.408 µL	612.04 µL	1.224 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	816.94 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Conjugation of Fluorochromes to Monoclonal Antibodies.

Authors:

Lantz, Larry and Holmes, Kevin and Douagi, Iyadh

Journal:

Current protocols (2023): e795

Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase.

Authors:

Arantes, Thales Domingos and Theodoro, Raquel Cordeiro and Teixeira, Marcus de Melo and Bagagli, Eduardo

Journal:

Memorias do Instituto Oswaldo Cruz (2017): 140-145

Preparation of fluorescent labeled gentamicin as biological tracer and its characterization by liquid chromatography and high resolution mass spectrometry.

Authors:

Woiwode, Ulrich and Sievers-Engler, Adrian and Lämmerhofer, Michael

Journal:

Journal of pharmaceutical and biomedical analysis (2016): 307-315

Development of a screening fluorescence polarization immunoassay for the simultaneous detection of fumonisins B₁ and B₂ in maize.

Authors:

Li, Chenglong and Mi, Tiejun and Conti, Gea Oliveri and Yu, Qing and Wen, Kai and Shen, Jianzhong and Ferrante, Margherita and Wang, Zhanhui

Journal:

Journal of agricultural and food chemistry (2015): 4940-6

Interruption of lung cancer cell migration and proliferation by fungal immunomodulatory protein FIP-fve from Flammulina velutipes.

Authors:

Chang, Yu-Chi and Hsiao, Yi-Min and Wu, Ming-Fang and Ou, Chu-Chyn and Lin, Yu-Wen and Lue, Ko-Huang and Ko, Jiunn-Liang

Journal:

Journal of agricultural and food chemistry (2013): 12044-52

Physical properties

Molecular weight	816.94
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.227
Correction factor (280 nm)	0.203
Extinction coefficient (cm ^-1 M ^-1)	67000 ¹
Excitation (nm)	595
Emission (nm)	613
Quantum yield	0.07 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	188828-09-3

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Flow Cytometry Fluorochromes

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Page updated on December 15, 2025