Texas Red-X, succinimidyl ester Mixed isomers CAS 216972-99-5

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Physical properties

Molecular weight	816.94
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5*

Overview SDS Protocol

CAS

216972-99-5

Molecular weight

816.94

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.97¹, 0.93²

The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen). It has the same spectral properties to Texas Red, but it is much easier conjugated to a biomolecure with much higher conjugation yield. It can be used to create bright red-fluorescent bioconjugates with excitation/emission maxima ~595/615 nm. Compared to Texas Red®, this reactive dye contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the succinimidyl ester group. This spacer helps to separate the fluorophore from its point of attachment, potentially reducing the interaction of the fluorophore with the biomolecule to which it is conjugated. This product contains the mixed 2- and 5-isomers with a variable batch-dependent ratio. We also offer the single 5-isomer (#474) that gives more reproducible results for critical productions.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.408 µL	612.04 µL	1.224 mL	6.12 mL	12.241 mL
5 mM	24.482 µL	122.408 µL	244.816 µL	1.224 mL	2.448 mL
10 mM	12.241 µL	61.204 µL	122.408 µL	612.04 µL	1.224 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Images

Figure 1. Chemical structure for Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5*

Citations

View all 1 citations: Citation Explorer

Glycosylation of a key cubilin Asn residue results in reduced binding to albumin
Authors: Yadav, Shiv Pratap Singh and Yu, Aiying and Zhao, Jingfu and Singh, Jasdeep and Kakkar, Saloni and Chakraborty, Srinivas and Mechref, Yehia and Molitoris, Bruce and Wagner, Mark C
Journal: Journal of Biological Chemistry (2022): 102371

References

View all 63 references: Citation Explorer

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Journal: J Exp Biol (2005): 4363

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Journal: Appl Environ Microbiol (2005): 6453

Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
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Journal: Biophys J (2005): 3455

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
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Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
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Journal: Shock (2003): 582