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Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5*

Chemical structure for Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5*
Chemical structure for Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5*
Ordering information
Price ()
Catalog Number475
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight816.94
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
216972-99-5
Molecular weight
816.94
Correction Factor (260 nm)
0.23
Correction Factor (280 nm)
0.36
Correction Factor (565 nm)
0.4472
Extinction coefficient (cm -1 M -1)
1160001
Excitation (nm)
586
Emission (nm)
603
Quantum yield
0.931, 0.352
The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen). It has the same spectral properties to Texas Red, but it is much easier conjugated to a biomolecure with much higher conjugation yield. It can be used to create bright red-fluorescent bioconjugates with excitation/emission maxima ~595/615 nm. Compared to Texas Red®, this reactive dye contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the succinimidyl ester group. This spacer helps to separate the fluorophore from its point of attachment, potentially reducing the interaction of the fluorophore with the biomolecule to which it is conjugated. This product contains the mixed 2- and 5-isomers with a variable batch-dependent ratio. We also offer the single 5-isomer (#474) that gives more reproducible results for critical productions.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.408 µL612.04 µL1.224 mL6.12 mL12.241 mL
5 mM24.482 µL122.408 µL244.816 µL1.224 mL2.448 mL
10 mM12.241 µL61.204 µL122.408 µL612.04 µL1.224 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352

Citations


View all 1 citations: Citation Explorer
Glycosylation of a key cubilin Asn residue results in reduced binding to albumin
Authors: Yadav, Shiv Pratap Singh and Yu, Aiying and Zhao, Jingfu and Singh, Jasdeep and Kakkar, Saloni and Chakraborty, Srinivas and Mechref, Yehia and Molitoris, Bruce and Wagner, Mark C
Journal: Journal of Biological Chemistry (2022): 102371

References


View all 63 references: Citation Explorer
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Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
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