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Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5*

Chemical structure for Texas Red-X, succinimidyl ester *Single isomer* *CAS 178623-11-5*
Chemical structure for Texas Red-X, succinimidyl ester *Single isomer* *CAS 178623-11-5*
Ordering information
Price ()
Catalog Number474
Unit Size
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Additional ordering information
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Fax1-408-733-1304
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Physical properties
Molecular weight816.94
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
178623-11-5
Molecular weight
816.94
Correction Factor (260 nm)
0.23
Correction Factor (280 nm)
0.36
Correction Factor (565 nm)
0.4472
Extinction coefficient (cm -1 M -1)
1160001
Excitation (nm)
586
Emission (nm)
603
Quantum yield
0.931, 0.352
The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen). It has the same spectral properties to Texas Red, but it is much easier conjugated to a biomolecure with much higher conjugation yield. It can be used to create bright red-fluorescent bioconjugates with excitation/emission maxima ~595/615 nm. Compared to Texas Red®, this reactive dye contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the succinimidyl ester group. This spacer helps to separate the fluorophore from its point of attachment, potentially reducing the interaction of the fluorophore with the biomolecule to which it is conjugated. Compared to the mixed isomers (#474), this product is the single 5-isomer, giving more reproducible results.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.408 µL612.04 µL1.224 mL6.12 mL12.241 mL
5 mM24.482 µL122.408 µL244.816 µL1.224 mL2.448 mL
10 mM12.241 µL61.204 µL122.408 µL612.04 µL1.224 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352

Citations


View all 5 citations: Citation Explorer
Glycosylation of a key cubilin Asn residue results in reduced binding to albumin
Authors: Yadav, Shiv Pratap Singh and Yu, Aiying and Zhao, Jingfu and Singh, Jasdeep and Kakkar, Saloni and Chakraborty, Srinivas and Mechref, Yehia and Molitoris, Bruce and Wagner, Mark C
Journal: Journal of Biological Chemistry (2022): 102371
A Novel Active Peptide from Rapana venosa Protects Against Gentamicin-Induced Sensory Hair Cell Loss in Zebrafish
Authors: Gao, Yan and Zhang, Shanshan and Wan, Jiahui and Wang, Rongchun and Ba, Shuaikang and Ji, Xiuna and Sun, Chen and Liu, Kechun
Journal: International Journal of Peptide Research and Therapeutics (2020): 1--9
A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos
Authors: Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili
Journal: Journal of Applied Toxicology (2020)
Mechanism of How Carbamylation Reduces Albumin Binding to FcRn contributing to increased vascular clearance.
Authors: Yadav, Shiv Pratap S and Sandoval, Ruben and Zhao, Jingfu and Huang, Yifan and Wang, Exing and Kumar, Sudhanshu and Campos-Bilderback, Silvia B and Rhodes, George J and Mechref, Yehia and Molitoris, Bruce A and others,
Journal: American Journal of Physiology-Renal Physiology (2020)
Alternative enzyme protection assay to overcome the drawbacks of the gentamicin protection assay for measuring entry and intracellular survival of staphylococci
Authors: Kim, Jin-Hahn and Chaurasia, Akhilesh Kumar and Batool, Nayab and Ko, Kwan Soo and Kim, Kyeong Kyu
Journal: Infection and immunity (2019): e00119--19

References


View all 63 references: Citation Explorer
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Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
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Purification and fluorescent labeling of the human serotonin transporter
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Budding dynamics of multicomponent tubular vesicles
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Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
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Journal: Appl Environ Microbiol (2005): 6453
Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455
Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
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Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
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