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Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5*

The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen). It has the same spectral properties to Texas Red, but it is much easier conjugated to a biomolecure with much higher conjugation yield. It can be used to create bright red-fluorescent bioconjugates with excitation/emission maxima ~595/615 nm. Compared to Texas Red®, this reactive dye contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the succinimidyl ester group. This spacer helps to separate the fluorophore from its point of attachment, potentially reducing the interaction of the fluorophore with the biomolecule to which it is conjugated. Compared to the mixed isomers (#474), this product is the single 5-isomer, giving more reproducible results.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.408 µL612.04 µL1.224 mL6.12 mL12.241 mL
5 mM24.482 µL122.408 µL244.816 µL1.224 mL2.448 mL
10 mM12.241 µL61.204 µL122.408 µL612.04 µL1.224 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Citations

View all 5 citations: Citation Explorer
Glycosylation of a key cubilin Asn residue results in reduced binding to albumin
Authors: Yadav, Shiv Pratap Singh and Yu, Aiying and Zhao, Jingfu and Singh, Jasdeep and Kakkar, Saloni and Chakraborty, Srinivas and Mechref, Yehia and Molitoris, Bruce and Wagner, Mark C
Journal: Journal of Biological Chemistry (2022): 102371
A Novel Active Peptide from Rapana venosa Protects Against Gentamicin-Induced Sensory Hair Cell Loss in Zebrafish
Authors: Gao, Yan and Zhang, Shanshan and Wan, Jiahui and Wang, Rongchun and Ba, Shuaikang and Ji, Xiuna and Sun, Chen and Liu, Kechun
Journal: International Journal of Peptide Research and Therapeutics (2020): 1--9
A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos
Authors: Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili
Journal: Journal of Applied Toxicology (2020)
Mechanism of How Carbamylation Reduces Albumin Binding to FcRn contributing to increased vascular clearance.
Authors: Yadav, Shiv Pratap S and Sandoval, Ruben and Zhao, Jingfu and Huang, Yifan and Wang, Exing and Kumar, Sudhanshu and Campos-Bilderback, Silvia B and Rhodes, George J and Mechref, Yehia and Molitoris, Bruce A and others,
Journal: American Journal of Physiology-Renal Physiology (2020)
Alternative enzyme protection assay to overcome the drawbacks of the gentamicin protection assay for measuring entry and intracellular survival of staphylococci
Authors: Kim, Jin-Hahn and Chaurasia, Akhilesh Kumar and Batool, Nayab and Ko, Kwan Soo and Kim, Kyeong Kyu
Journal: Infection and immunity (2019): e00119--19

References

View all 63 references: Citation Explorer
Bead-based cellular analysis, sorting and multiplexing
Authors: Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: Chembiochem (2005): 1341
Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455
Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453
Budding dynamics of multicomponent tubular vesicles
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996
Purification and fluorescent labeling of the human serotonin transporter
Authors: Rasmussen SG, Gether U.
Journal: Biochemistry (2005): 3494
Page updated on November 7, 2024

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Physical properties

Molecular weight

816.94

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm -1 M -1)

1160001

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.971, 0.932

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

178623-11-5