Texas Red-X, succinimidyl ester Single isomer 178623-11-5

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Physical properties

Molecular weight	816.94
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Texas Red-X, succinimidyl ester *Mixed isomers* *CAS 216972-99-5*

Overview SDS Protocol

CAS

178623-11-5

Molecular weight

816.94

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.97¹, 0.93²

The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen). It has the same spectral properties to Texas Red, but it is much easier conjugated to a biomolecure with much higher conjugation yield. It can be used to create bright red-fluorescent bioconjugates with excitation/emission maxima ~595/615 nm. Compared to Texas Red®, this reactive dye contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the succinimidyl ester group. This spacer helps to separate the fluorophore from its point of attachment, potentially reducing the interaction of the fluorophore with the biomolecule to which it is conjugated. Compared to the mixed isomers (#474), this product is the single 5-isomer, giving more reproducible results.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.408 µL	612.04 µL	1.224 mL	6.12 mL	12.241 mL
5 mM	24.482 µL	122.408 µL	244.816 µL	1.224 mL	2.448 mL
10 mM	12.241 µL	61.204 µL	122.408 µL	612.04 µL	1.224 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Images

Figure 1. Chemical structure for Texas Red-X, succinimidyl ester *Single isomer* *CAS 178623-11-5*

Citations

View all 5 citations: Citation Explorer

Glycosylation of a key cubilin Asn residue results in reduced binding to albumin
Authors: Yadav, Shiv Pratap Singh and Yu, Aiying and Zhao, Jingfu and Singh, Jasdeep and Kakkar, Saloni and Chakraborty, Srinivas and Mechref, Yehia and Molitoris, Bruce and Wagner, Mark C
Journal: Journal of Biological Chemistry (2022): 102371

A Novel Active Peptide from Rapana venosa Protects Against Gentamicin-Induced Sensory Hair Cell Loss in Zebrafish
Authors: Gao, Yan and Zhang, Shanshan and Wan, Jiahui and Wang, Rongchun and Ba, Shuaikang and Ji, Xiuna and Sun, Chen and Liu, Kechun
Journal: International Journal of Peptide Research and Therapeutics (2020): 1--9

A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos
Authors: Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili
Journal: Journal of Applied Toxicology (2020)

Mechanism of How Carbamylation Reduces Albumin Binding to FcRn contributing to increased vascular clearance.
Authors: Yadav, Shiv Pratap S and Sandoval, Ruben and Zhao, Jingfu and Huang, Yifan and Wang, Exing and Kumar, Sudhanshu and Campos-Bilderback, Silvia B and Rhodes, George J and Mechref, Yehia and Molitoris, Bruce A and others,
Journal: American Journal of Physiology-Renal Physiology (2020)

Alternative enzyme protection assay to overcome the drawbacks of the gentamicin protection assay for measuring entry and intracellular survival of staphylococci
Authors: Kim, Jin-Hahn and Chaurasia, Akhilesh Kumar and Batool, Nayab and Ko, Kwan Soo and Kim, Kyeong Kyu
Journal: Infection and immunity (2019): e00119--19

References

View all 63 references: Citation Explorer

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A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
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Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
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Journal: J Exp Biol (2005): 4363

Purification and fluorescent labeling of the human serotonin transporter
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Budding dynamics of multicomponent tubular vesicles
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Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
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Journal: Appl Environ Microbiol (2005): 6453

Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
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Journal: Biomacromolecules (2004): 2347

Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
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