Name: TF1-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17006
Price: 348 USD
Availability: InStock

TF1-dUTP

1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TF1-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua™ filter set. (Spectrum Aqua™ is the trademark of Vysis).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17006	25 nmoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of TF1-dUTP *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.347 µL	536.734 µL	1.073 mL	5.367 mL	10.735 mL
5 mM	21.469 µL	107.347 µL	214.694 µL	1.073 mL	2.147 mL
10 mM	10.735 µL	53.673 µL	107.347 µL	536.734 µL	1.073 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	931.56 g/mol	=	mL	x	mM	=	mmol

References

View all 9 references: Citation Explorer

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1

Authors:

Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.

Journal:

Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

Authors:

Cox WG, Singer VL.

Journal:

Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX

Authors:

Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.

Journal:

Bioconjug Chem (2003): 919

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking

Authors:

Wooddell CI, Burgess RR.

Journal:

Biochemistry (2000): 13405

Simple method for preparation of fluor/hapten-labeled dUTP

Authors:

Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.

Journal:

Biotechniques (2000): 518

Physical properties

Molecular weight	931.56
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	35000
Excitation (nm)	420
Emission (nm)	461

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 18, 2025