Thiazole orange *CAS 107091-89-4*

Thiazole orange

CAS 107091-89-4

Thiazole orange is a fluorescent DNA-binding dye commonly used to analyze reticulocytes and Plasmodium species. Its derivatives—SYBR Green, PicoGreen, TOTO, and YOYO—are widely used for detecting DNA samples, enhancing sensitivity and accuracy in applications like PCR assays and gel electrophoresis.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17518	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thiazole orange *CAS 107091-89-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	209.815 µL	1.049 mL	2.098 mL	10.491 mL	20.982 mL
5 mM	41.963 µL	209.815 µL	419.63 µL	2.098 mL	4.196 mL
10 mM	20.982 µL	104.908 µL	209.815 µL	1.049 mL	2.098 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	476.61 g/mol	=	mL	x	mM	=	mmol

References

View all 52 references: Citation Explorer

Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode

Authors:

Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.

Journal:

J Am Chem Soc (2006): 2954

Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage

Authors:

Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.

Journal:

Photochem Photobiol. (2006)

Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties

Authors:

Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.

Journal:

Transfusion (2006): 213

Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization

Authors:

Algar WR, Massey M, Krull UJ.

Journal:

J Fluoresc (2006): 555

Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange

Authors:

Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.

Journal:

Chemistry. (2006)

Physical properties

Molecular weight	476.61
Solvent	DMSO

Spectral properties

Excitation (nm)	514
Emission (nm)	533

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	107091-89-4

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