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Thiazole orange *CAS 107091-89-4*

Thiazole orange is a fluorescent DNA-binding dye commonly used to analyze reticulocytes and Plasmodium species. Its derivatives—SYBR Green, PicoGreen, TOTO, and YOYO—are widely used for detecting DNA samples, enhancing sensitivity and accuracy in applications like PCR assays and gel electrophoresis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thiazole orange *CAS 107091-89-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM209.815 µL1.049 mL2.098 mL10.491 mL20.982 mL
5 mM41.963 µL209.815 µL419.63 µL2.098 mL4.196 mL
10 mM20.982 µL104.908 µL209.815 µL1.049 mL2.098 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Acridine orange49052027000
Acridine orange *10 mg/mL solution in water*49052027000
CytoTell™ Orange541560-
LysoBrite™ Orange543565-

References

View all 52 references: Citation Explorer
Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
Page updated on October 12, 2024

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Physical properties

Molecular weight

476.61

Solvent

DMSO

Spectral properties

Excitation (nm)

514

Emission (nm)

533

Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134

CAS

107091-89-4
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