Thioflavin T | AAT Bioquest

Thioflavin T, succinimidyl ester

Thioflavin T (ThT), also called Basic Yellow 1 or CI 49005, is a benzothiazole salt. The dye is used to visualize plaques composed of beta-amyloid found in the brains of Alzheimer's disease patients. When it binds to beta sheets, such as those in amyloid oligomers, the dye undergoes a characteristic 120 nm red shift of its excitation spectrum that may be selectively excited at 450 nm, resulting in a fluorescence signal at 482 nm. ThT binds rapidly and specifically to the anti-parallel beta-sheet fibrils formed from synthetic beta-amyloid (1-40), but does not bind to monomer or oligomeric intermediates. The binding of ThT does not interfere with the aggregation of beta-amyloid peptide into amyloid fibrils. Thioflavin T, succinimidyl ester is a valuble tool for making various bioconjugates of Thioflavin T that can direct Thioflavin T to a desired target. It is an essential reagent to make the peptides and other conjugates of Thioflavin T.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23065	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thioflavin T, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	176.239 µL	881.197 µL	1.762 mL	8.812 mL	17.624 mL
5 mM	35.248 µL	176.239 µL	352.479 µL	1.762 mL	3.525 mL
10 mM	17.624 µL	88.12 µL	176.239 µL	881.197 µL	1.762 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	567.41 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Human Telomeric Hybrid-2-over-Hybrid-1 G-quadruplex Targeting and a Selective Hypersaline-Tolerant Sensor Using Abasic Site-Engineered Monomorphism

Authors:

Wu, Tao and Ye, Meiyun and Mao, Tianyi and Lin, Fan and Hu, Yuehua and Gan, Ning and Shao, Yong

Journal:

Analytica Chimica Acta (2017)

Fluorescent dyes in the context of DNA-binding: The case of Thioflavin T

Authors:

Biancardi, Aless and ro , undefined and Biver, Tarita and Mennucci, Benedetta

Journal:

International Journal of Quantum Chemistry (2017)

Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels

Authors:

Sugimoto, Shinya and Arita-Morioka, Ken-ichi and Mizunoe, Yoshimitsu and Yamanaka, Kunitoshi and Ogura, Teru

Journal:

Nucleic acids research (2015): gkv338

Determinants of amyloid fibril degradation by the PDZ protease HTRA1

Authors:

Poepsel, Simon and Sprengel, Andreas and Sacca, Barbara and Kaschani, Farnusch and Kaiser, Markus and Gatsogiannis, Christos and Raunser, Stefan and Clausen, Tim and Ehrmann, Michael

Journal:

Nature chemical biology (2015): 862--869

Mechanistic aspects of thioflavin-T self-aggregation and DNA binding: evidence for dimer attack on DNA grooves

Authors:

Biancardi, A and Biver, T and Burgalassi, A and Mattonai, M and Secco, F and Venturini, M

Journal:

Physical Chemistry Chemical Physics (2014): 20061--20072

References

View all 28 references: Citation Explorer

Methods for enhancing the accuracy and reproducibility of Congo red and thioflavin T assays

Authors:

Eisert R, Felau L, Brown LR.

Journal:

Anal Biochem (2006): 144

Characteristics of the binding of thioflavin S to tau paired helical filaments

Authors:

Santa-Maria I, Perez M, Hern and ez F, Avila J, Moreno FJ.

Journal:

J Alzheimers Dis (2006): 279

Mechanism of thioflavin T accumulation inside cells overexpressing P-glycoprotein or multidrug resistance-associated protein: role of lipophilicity and positive charge

Authors:

Darghal N, Garnier-Suillerot A, Salerno M.

Journal:

Biochem Biophys Res Commun (2006): 623

Oligo(ethylene glycol) derivatives of Thioflavin T as inhibitors of protein-amyloid interactions

Authors:

Inbar P, Li CQ, Takayama SA, Bautista MR, Yang J.

Journal:

Chembiochem (2006): 1563

Lipophilic analogs of thioflavin S as novel amyloid-imaging agents

Authors:

Wu C, Cai L, Wei J, Pike VW, Wang Y.

Journal:

Curr Alzheimer Res (2006): 259

Physical properties

Molecular weight	567.41
Solvent	DMSO

Spectral properties

Absorbance (nm)	199
Excitation (nm)	349
Emission (nm)	454

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 10, 2024