Thiolite™ Blue

Thiolite™ Blue is one of the most sensitive sensors for measuring thiol compounds. It gives a blue fluorescent adduct upon reacting with thiol compounds (such as GSH and cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds. Thiolite™ Blue is an excellent replacement for mBBr (monobromobimane) due to their similar spectral properties. Compared to mBBr, the thiol adduct of Thiolite™ Blue has much stonger fluorescence and absorption than that of mBBr, making it a much more sensitive thiol probe than bromobimanes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21507	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thiolite™ Blue to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	345.734 µL	1.729 mL	3.457 mL	17.287 mL	34.573 mL
5 mM	69.147 µL	345.734 µL	691.467 µL	3.457 mL	6.915 mL
10 mM	34.573 µL	172.867 µL	345.734 µL	1.729 mL	3.457 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	289.24 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans

Authors:

Xiao, Man and Du, Guankui and Zhong, Guobing and Yan, Dongjing and Zeng, Huazong and Cai, Wangwei

Journal:

PloS one (2016): e0151103

Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells

Authors:

Niikura, Kenichi and Sugimura, Naotoshi and Musashi, Yusuke and Mikuni, Shintaro and Matsuo, Yasutaka and Kobayashi, Shintaro and Nagakawa, Keita and Takahara, Shuko and Takeuchi, Chie and Sawa, Hirofumi and others, undefined

Journal:

Molecular biosystems (2013): 501--507

References

View all 74 references: Citation Explorer

Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events

Authors:

Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.

Journal:

Anal Chem (2012): 194

Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids

Authors:

Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.

Journal:

Anal Chem. (2012)

A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma

Authors:

Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132

Investigation of chemically modified barium titanate beads as surface-enhanced Raman scattering (SERS) active substrates for the detection of benzene thiol, 1,2-benzene dithiol, and rhodamine 6G

Authors:

Onuegbu J, Fu A, Glembocki O, Pokes S, Alexson D, Hosten CM.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2011): 456

Simple and rapid determination of thiol compounds by HPLC and fluorescence detection with 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide) difluoroboradiaza-s-indacene

Authors:

Guo XF, Zhao PX, Wang H, Zhang HS.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932

Physical properties

Molecular weight	289.24
Solvent	DMSO

Spectral properties

Excitation (nm)	335
Emission (nm)	460

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025