Thiolite™ Blue

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10010.1- Dose-ResponseData legend Generated with Quest Graphâ„¢ GSH (uM) RFU Hover mouse to interact
GSH dose responses were measured on a solid black 96-well plate with Cat#21507 Thiolite™ Blue.
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Unit Size: Cat No: Price (USD): Qty:
5 mg 21507 $95


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)335/460
MW289.24
SolventDMSO
Storage F/D/L
Category Neurobiology
Reactive Oxygen Species
Related Cell Functional Analysis
Redox Enzymes
Thiolite™ Blue is one of the most sensitive sensors for measuring thiol compounds. It gives a blue fluorescent adduct upon reacting with thiol compounds (such as GSH and cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds. Thiolite™ Blue is an excellent replacement for mBBr (monobromobimane) due to their similar spectral properties. Compared to mBBr, the thiol adduct of Thiolite™ Blue has much stonger fluorescence and absorption than that of mBBr, making it a much more sensitive thiol probe than bromobimanes.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thiolite™ Blue to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

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Wavelength (nm)





References & Citations

Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans
Authors: Man Xiao, Guankui Du, Guobing Zhong, Dongjing Yan, Huazong Zeng, Wangwei Cai
Journal: PloS one (2016): e0151103

Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells
Authors: Kenichi Niikura, Naotoshi Sugimura, Yusuke Musashi, Shintaro Mikuni, Yasutaka Matsuo, Shintaro Kobayashi, Keita Nagakawa, Shuko Takahara, Chie Takeuchi, Hirofumi Sawa
Journal: Molecular biosystems (2013): 501--507






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Cell Apoptosis & Proliferation

Certificate of Analysis