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Thiolite™ Blue
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Thiolite™ Blue is one of the most sensitive sensors for measuring thiol compounds. It gives a blue fluorescent adduct upon reacting with thiol compounds (such as GSH and cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds. Thiolite™ Blue is an excellent replacement for mBBr (monobromobimane) due to their similar spectral properties. Compared to mBBr, the thiol adduct of Thiolite™ Blue has much stonger fluorescence and absorption than that of mBBr, making it a much more sensitive thiol probe than bromobimanes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Thiolite™ Blue to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 345.734 µL | 1.729 mL | 3.457 mL | 17.287 mL | 34.573 mL |
| 5 mM | 69.147 µL | 345.734 µL | 691.467 µL | 3.457 mL | 6.915 mL |
| 10 mM | 34.573 µL | 172.867 µL | 345.734 µL | 1.729 mL | 3.457 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Thiolite™ Green | 505 | 524 |
Citations
View all 2 citations: Citation Explorer
Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans
Authors: Xiao, Man and Du, Guankui and Zhong, Guobing and Yan, Dongjing and Zeng, Huazong and Cai, Wangwei
Journal: PloS one (2016): e0151103
Authors: Xiao, Man and Du, Guankui and Zhong, Guobing and Yan, Dongjing and Zeng, Huazong and Cai, Wangwei
Journal: PloS one (2016): e0151103
Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells
Authors: Niikura, Kenichi and Sugimura, Naotoshi and Musashi, Yusuke and Mikuni, Shintaro and Matsuo, Yasutaka and Kobayashi, Shintaro and Nagakawa, Keita and Takahara, Shuko and Takeuchi, Chie and Sawa, Hirofumi and others, undefined
Journal: Molecular biosystems (2013): 501--507
Authors: Niikura, Kenichi and Sugimura, Naotoshi and Musashi, Yusuke and Mikuni, Shintaro and Matsuo, Yasutaka and Kobayashi, Shintaro and Nagakawa, Keita and Takahara, Shuko and Takeuchi, Chie and Sawa, Hirofumi and others, undefined
Journal: Molecular biosystems (2013): 501--507
References
View all 74 references: Citation Explorer
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
Authors: Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.
Journal: Anal Chem (2012): 194
Authors: Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.
Journal: Anal Chem (2012): 194
Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids
Authors: Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.
Journal: Anal Chem. (2012)
Authors: Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.
Journal: Anal Chem. (2012)
A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma
Authors: Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132
Authors: Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132
Investigation of chemically modified barium titanate beads as surface-enhanced Raman scattering (SERS) active substrates for the detection of benzene thiol, 1,2-benzene dithiol, and rhodamine 6G
Authors: Onuegbu J, Fu A, Glembocki O, Pokes S, Alexson D, Hosten CM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2011): 456
Authors: Onuegbu J, Fu A, Glembocki O, Pokes S, Alexson D, Hosten CM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2011): 456
Simple and rapid determination of thiol compounds by HPLC and fluorescence detection with 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide) difluoroboradiaza-s-indacene
Authors: Guo XF, Zhao PX, Wang H, Zhang HS.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932
Authors: Guo XF, Zhao PX, Wang H, Zhang HS.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932