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Thiolite™ Blue

GSH dose responses were measured on a solid black 96-well plate with Cat#21507 Thiolite™ Blue.
GSH dose responses were measured on a solid black 96-well plate with Cat#21507 Thiolite™ Blue.
GSH dose responses were measured on a solid black 96-well plate with Cat#21507 Thiolite™ Blue.
Ordering information
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ShippingStandard overnight for United States, inquire for international
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Physical properties
Molecular weight289.24
Spectral properties
Excitation (nm)335
Emission (nm)460
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
Thiolite™ Green
Related products
Thiolite™ Blue, AM


Molecular weight
Excitation (nm)
Emission (nm)
Thiolite™ Blue is one of the most sensitive sensors for measuring thiol compounds. It gives a blue fluorescent adduct upon reacting with thiol compounds (such as GSH and cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds. Thiolite™ Blue is an excellent replacement for mBBr (monobromobimane) due to their similar spectral properties. Compared to mBBr, the thiol adduct of Thiolite™ Blue has much stonger fluorescence and absorption than that of mBBr, making it a much more sensitive thiol probe than bromobimanes.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thiolite™ Blue to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM345.734 µL1.729 mL3.457 mL17.287 mL34.573 mL
5 mM69.147 µL345.734 µL691.467 µL3.457 mL6.915 mL
10 mM34.573 µL172.867 µL345.734 µL1.729 mL3.457 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Excitation (nm)335
Emission (nm)460

Product Family

NameExcitation (nm)Emission (nm)
Thiolite™ Green505524



View all 2 citations: Citation Explorer
Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans
Authors: Xiao, Man and Du, Guankui and Zhong, Guobing and Yan, Dongjing and Zeng, Huazong and Cai, Wangwei
Journal: PloS one (2016): e0151103
Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells
Authors: Niikura, Kenichi and Sugimura, Naotoshi and Musashi, Yusuke and Mikuni, Shintaro and Matsuo, Yasutaka and Kobayashi, Shintaro and Nagakawa, Keita and Takahara, Shuko and Takeuchi, Chie and Sawa, Hirofumi and others, undefined
Journal: Molecular biosystems (2013): 501--507


View all 74 references: Citation Explorer
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
Authors: Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.
Journal: Anal Chem (2012): 194
Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids
Authors: Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.
Journal: Anal Chem. (2012)
A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma
Authors: Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132
Investigation of chemically modified barium titanate beads as surface-enhanced Raman scattering (SERS) active substrates for the detection of benzene thiol, 1,2-benzene dithiol, and rhodamine 6G
Authors: Onuegbu J, Fu A, Glembocki O, Pokes S, Alexson D, Hosten CM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2011): 456
Simple and rapid determination of thiol compounds by HPLC and fluorescence detection with 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide) difluoroboradiaza-s-indacene
Authors: Guo XF, Zhao PX, Wang H, Zhang HS.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932
Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier
Authors: Hu B, Zhao Y, Zhu HZ, Yu SH.
Journal: ACS Nano (2011): 3166
Thiol redox proteomics seen with fluorescent eyes: the detection of cysteine oxidative modifications by fluorescence derivatization and 2-DE
Authors: Izquierdo-Alvarez A, Martinez-Ruiz A.
Journal: J Proteomics (2011): 329
Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma
Authors: Huo FJ, Yang YT, Su J, Sun YQ, Yin CX, Yan XX.
Journal: Analyst (2011): 1892
Thiol-exchange in DTSSP crosslinked peptides is proportional to cysteine content and precisely controlled in crosslink detection by two-step LC-MALDI MSMS
Authors: Lambert W, Soderberg CA, Rutsdottir G, Boelens WC, Emanuelsson C.
Journal: Protein Sci (2011): 1682
Modulating DNA-templated silver nanoclusters for fluorescence turn-on detection of thiol compounds
Authors: Huang Z, Pu F, Lin Y, Ren J, Qu X.
Journal: Chem Commun (Camb) (2011): 3487