Thiolite™ Green

Thiolite™ Green is one of the most sensitive sensors for measuring thiol compounds. It gives a green fluorescent adduct upon reacting with thiol compounds (such as cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21508	5 mg	Price

Citations

View all 8 citations: Citation Explorer

Carboranyl-1, 8-naphthalimide intercalators induce lysosomal membrane permeabilization and ferroptosis in cancer cell lines

Authors:

Rykowski, Sebastian and Gurda-Wo{\'z}na, Dorota and Fedoruk-Wyszomirska, Agnieszka and Orlicka-P{\l}ocka, Marta and Kowalczyk, Aleksandra and St{\k{a}}czek, Pawe{\l} and Denel-Bobrowska, Marta and Biniek-Antosiak, Katarzyna and Rypniewski, Wojciech and Wyszko, Eliza and others,

Journal:

Journal of Enzyme Inhibition and Medicinal Chemistry (2023): 2171028

Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives

Authors:

Orlicka-P{\l}ocka, Marta and Fedoruk-Wyszomirska, Agnieszka and Gurda-Wo{\'z}na, Dorota and Pawelczak, Pawe{\l} and Krawczyk, Patrycja and Giel-Pietraszuk, Ma{\l}gorzata and Framski, Grzegorz and Ostrowski, Tomasz and Wyszko, Eliza

Journal:

Antioxidants (2021): 950

Revealing 3D structure of gluten in wheat dough by optical clearing imaging

Authors:

Ogawa, Takenobu and Matsumura, Yasuki

Journal:

Nature communications (2021): 1--10

Circumventing the Crabtree effect: forcing oxidative phosphorylation (OXPHOS) via galactose medium increases sensitivity of HepG2 cells to the purine derivative kinetin riboside

Authors:

Orlicka-P{\l}ocka, Marta and Gurda-Wozna, Dorota and Fedoruk-Wyszomirska, Agnieszka and Wyszko, Eliza

Journal:

Apoptosis (2020): 835--852

Quercetin Triggers Induction of Apoptotic and Lysosomal Death of Sensitive and Multidrug Resistant Leukaemia HL60 Cells

Authors:

Maruszewska, Agnieszka and Tarasiuk, Jolanta

Journal:

Nutrition and Cancer (2020): 1--18

References

View all 74 references: Citation Explorer

Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events

Authors:

Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.

Journal:

Anal Chem (2012): 194

Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids

Authors:

Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.

Journal:

Anal Chem. (2012)

A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma

Authors:

Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132

Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier

Authors:

Hu B, Zhao Y, Zhu HZ, Yu SH.

Journal:

ACS Nano (2011): 3166

Simple and rapid determination of thiol compounds by HPLC and fluorescence detection with 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide) difluoroboradiaza-s-indacene

Authors:

Guo XF, Zhao PX, Wang H, Zhang HS.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932

Physical properties

Molecular weight	~400
Solvent	DMSO

Spectral properties

Excitation (nm)	505
Emission (nm)	524

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 29, 2025