AAT Bioquest

Thiolite™ Green

Spectrum for Thiolite Green
Spectrum for Thiolite Green
Spectrum for Thiolite Green
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight~400
Spectral properties
Excitation (nm)505
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
Thiolite™ Blue
Related products
Thiolite™ Blue, AM


Molecular weight
Excitation (nm)
Emission (nm)
Thiolite™ Green is one of the most sensitive sensors for measuring thiol compounds. It gives a green fluorescent adduct upon reacting with thiol compounds (such as cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds.


Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)505
Emission (nm)524

Product Family

NameExcitation (nm)Emission (nm)
Thiolite™ Blue335460



View all 8 citations: Citation Explorer
Carboranyl-1, 8-naphthalimide intercalators induce lysosomal membrane permeabilization and ferroptosis in cancer cell lines
Authors: Rykowski, Sebastian and Gurda-Wo{\'z}na, Dorota and Fedoruk-Wyszomirska, Agnieszka and Orlicka-P{\l}ocka, Marta and Kowalczyk, Aleksandra and St{\k{a}}czek, Pawe{\l} and Denel-Bobrowska, Marta and Biniek-Antosiak, Katarzyna and Rypniewski, Wojciech and Wyszko, Eliza and others,
Journal: Journal of Enzyme Inhibition and Medicinal Chemistry (2023): 2171028
Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives
Authors: Orlicka-P{\l}ocka, Marta and Fedoruk-Wyszomirska, Agnieszka and Gurda-Wo{\'z}na, Dorota and Pawelczak, Pawe{\l} and Krawczyk, Patrycja and Giel-Pietraszuk, Ma{\l}gorzata and Framski, Grzegorz and Ostrowski, Tomasz and Wyszko, Eliza
Journal: Antioxidants (2021): 950
Revealing 3D structure of gluten in wheat dough by optical clearing imaging
Authors: Ogawa, Takenobu and Matsumura, Yasuki
Journal: Nature communications (2021): 1--10
Circumventing the Crabtree effect: forcing oxidative phosphorylation (OXPHOS) via galactose medium increases sensitivity of HepG2 cells to the purine derivative kinetin riboside
Authors: Orlicka-P{\l}ocka, Marta and Gurda-Wozna, Dorota and Fedoruk-Wyszomirska, Agnieszka and Wyszko, Eliza
Journal: Apoptosis (2020): 835--852
Quercetin Triggers Induction of Apoptotic and Lysosomal Death of Sensitive and Multidrug Resistant Leukaemia HL60 Cells
Authors: Maruszewska, Agnieszka and Tarasiuk, Jolanta
Journal: Nutrition and Cancer (2020): 1--18
The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function
Authors: Wu, Tsung-Han and Yeh, Kun-Yun and Wang, Chen-Hsu and Wang, Hang and Li, Tsung-Lin and Chan, Yi-Lin and Wu, Chang-Jer
Journal: Journal of oncology (2019)
Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans
Authors: Xiao, Man and Du, Guankui and Zhong, Guobing and Yan, Dongjing and Zeng, Huazong and Cai, Wangwei
Journal: PloS one (2016): e0151103
Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells
Authors: Niikura, Kenichi and Sugimura, Naotoshi and Musashi, Yusuke and Mikuni, Shintaro and Matsuo, Yasutaka and Kobayashi, Shintaro and Nagakawa, Keita and Takahara, Shuko and Takeuchi, Chie and Sawa, Hirofumi and others, undefined
Journal: Molecular biosystems (2013): 501--507


View all 74 references: Citation Explorer
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
Authors: Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.
Journal: Anal Chem (2012): 194
Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids
Authors: Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.
Journal: Anal Chem. (2012)
A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma
Authors: Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132
Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier
Authors: Hu B, Zhao Y, Zhu HZ, Yu SH.
Journal: ACS Nano (2011): 3166
Simple and rapid determination of thiol compounds by HPLC and fluorescence detection with 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide) difluoroboradiaza-s-indacene
Authors: Guo XF, Zhao PX, Wang H, Zhang HS.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932
Thiol redox proteomics seen with fluorescent eyes: the detection of cysteine oxidative modifications by fluorescence derivatization and 2-DE
Authors: Izquierdo-Alvarez A, Martinez-Ruiz A.
Journal: J Proteomics (2011): 329
Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma
Authors: Huo FJ, Yang YT, Su J, Sun YQ, Yin CX, Yan XX.
Journal: Analyst (2011): 1892
Thiol-exchange in DTSSP crosslinked peptides is proportional to cysteine content and precisely controlled in crosslink detection by two-step LC-MALDI MSMS
Authors: Lambert W, Soderberg CA, Rutsdottir G, Boelens WC, Emanuelsson C.
Journal: Protein Sci (2011): 1682
Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition
Authors: Lin Y, Tao Y, Ren J, Pu F, Qu X.
Journal: Biosens Bioelectron (2011): 339
Investigation of chemically modified barium titanate beads as surface-enhanced Raman scattering (SERS) active substrates for the detection of benzene thiol, 1,2-benzene dithiol, and rhodamine 6G
Authors: Onuegbu J, Fu A, Glembocki O, Pokes S, Alexson D, Hosten CM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2011): 456