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ThiolTrace™ Violet 500
The subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. ThiolTrace™ Violet 500 is a brighter and more robust intracellular thiol probe for monitoring intracelluar GSH than the commonly used mBBr, mBCl or Thiotracker™ Violet. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTrace™ Violet 500 can be used in estimating the cellular level of reduced glutathione. It is at least 10X brighter than mBCL and other intracellular common thiol detection probes (e.g., Thiotracker Violet), and can be excited with UV or 405 nm excitation with a large Stokes shift. It can be fixed with aldehydes and permeabilized by Triton® X-100 (0.5%). It may be used in multiplex assays including cytotoxicity studies. ThiolTrace Violet 500 provides a simple, sensitive and reproducible tool to detect reduced GSH content in biological samples. ThiolTrace Violet 500 reacts with thiol to emit a strong fluorescence of 520-530 nm with excitation at the common violet 405 nm laser. When compared with ThiolTracker Violet (Thermo Fisher Scientific), ThiolTrace Violet 500 has 10-100 fold higher intensity in cell culture medium containing growth factors. ThiolTrace Violet 500 is compatible with wide variety of diluents including serum containing cell culture medium. ThiolTrace™ Violet 500 can be used for Flow Cytometry, HCS imaging and epifluorescent microscopy.
The comparison in the fluorescence intensity of ThiolTrace&trade; Violet 500 with ThiolTracker<sup>TM</sup> Violet (Thermo Scientific) in Jurkat cells in the <strong>presence of cell culture medium</strong>. Jurkat cells were dye loaded with ThiolTrace&trade; Violet 500 or ThiolTracker<sup>TM</sup> Violet for 20 minutes in a 37 <sup>o</sup>C, 5% CO<sub>2</sub> incubator. The fluorescence intensity was measured using ACEA NovoCyte 3000 flow cytometer with Pacific Orange channel.
The comparison in the fluorescence intensity of ThiolTrace&trade; Violet 500 with ThiolTracker<sup>TM</sup> Violet (Thermo Scientific) in Jurkat cells in the <strong>presence of cell culture medium</strong>. Jurkat cells were dye loaded with ThiolTrace&trade; Violet 500 or ThiolTracker<sup>TM</sup> Violet for 20 minutes in a 37 <sup>o</sup>C, 5% CO<sub>2</sub> incubator. The fluorescence intensity was measured using ACEA NovoCyte 3000 flow cytometer with Pacific Orange channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22280500 Tests
Price
 
Physical properties
Molecular weight557.01
SolventDMSO
Spectral properties
Excitation (nm)415
Emission (nm)499
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission525/50 nm filter
Instrument specification(s)Pacific Orange channel
Fluorescence microscope
Excitation405 nm
Emission525 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)TRITC filterset
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 29, 2025