logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Tide Fluor™ 1 CPG
TF1 CPG; 1000 Å; Superior replacement for EDANS
TF1 is designed to be a superior fluorophore alternative to EDANS. TF1 has (a). much stronger absorption; (b). much stronger fluorescence intensity; and (3). much less environment-sensitive fluorescence. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to EDANS. TF1-labeled peptides and nucleotides exhibit much stronger fluorescence and higher sensitivity than the ones labeled with EDANS. In pairing with our Tide Quencher™ 1 (TQ1), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.
<p style="background: white; margin: 6.0pt 0in 6.0pt 0in;"><span style="font-size: 10.0pt;">Oligonucleotide synthesis is carried out by a stepwise addition of nucleotide residues to the 5'-terminus of the growing chain until the desired sequence is assembled. Each addition is referred to as a synthetic cycle and consists of four chemical reactions: d<span class="mw-headline">e-blocking (detritylation)</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, coupling</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, capping</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, and oxidation</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">.</span></span></p>
<p style="background: white; margin: 6.0pt 0in 6.0pt 0in;"><span style="font-size: 10.0pt;">Oligonucleotide synthesis is carried out by a stepwise addition of nucleotide residues to the 5'-terminus of the growing chain until the desired sequence is assembled. Each addition is referred to as a synthetic cycle and consists of four chemical reactions: d<span class="mw-headline">e-blocking (detritylation)</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, coupling</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, capping</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, and oxidation</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">.</span></span></p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2241100 mg
Price
 
Physical properties
SolventMeCN
Spectral properties
Correction factor (280 nm)0.187
Extinction coefficient (cm -1 M -1)
20000
Excitation (nm)341
Emission (nm)447
Quantum yield
0.91
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 12, 2025