Tide Fluor™ 2 alkyne [TF2 alkyne]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 752.61 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 75000 |
Excitation (nm) | 503 |
Emission (nm) | 525 |
Quantum yield | 0.9 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
Tide Fluor™ 2 azide [TF2 azide] |
Related products
Overview | ![]() ![]() |
See also: Digital PCR, Fluoresceins, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, Tide Fluor Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 752.61 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 75000 | Excitation (nm) 503 | Emission (nm) 525 | Quantum yield 0.9 |
Tide Fluor™ 2 (TF2) family has the spectral properties similar to those of fluoresceins and Alexa Fluor® 488 (Alexa Fluor® is the trademark of Invitrogen). TF2 phosphoramidite enables the oligo labeling with the similar spectral properties and photostability of Alexa Fluor® 488 using the well established phosphoramidite chemistry. Compared to fluoresceins TF2 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluoresceins. TF2-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with FITC or FAM. In addition, TF2 is an excellent replacement for the expensive Alexa Fluor® 488 due to their similar spectral properties, pH dependence and photostability. In pairing with our Tide Quencher™ 2 (TQ2), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF2 product is reactive to azides, and useful for click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 2 alkyne [TF2 alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 132.871 µL | 664.355 µL | 1.329 mL | 6.644 mL | 13.287 mL |
5 mM | 26.574 µL | 132.871 µL | 265.742 µL | 1.329 mL | 2.657 mL |
10 mM | 13.287 µL | 66.435 µL | 132.871 µL | 664.355 µL | 1.329 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 75000 |
Excitation (nm) | 503 |
Emission (nm) | 525 |
Quantum yield | 0.9 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
Tide Fluor™ 1 alkyne [TF1 alkyne] | 341 | 448 | 20000 | 0.187 |
Tide Fluor™ 2 acid [TF2 acid] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
Tide Fluor™ 2 amine [TF2 amine] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
Tide Fluor™ 3 alkyne [TF3 alkyne] | 554 | 578 | 750001 | 0.179 |
Tide Fluor™ 5WS alkyne [TF5WS alkyne] | 649 | 664 | 250000 | 0.027 |
Tide Fluor™ 4 alkyne [TF4 alkyne] | 578 | 602 | 90000 | 0.436 |
Tide Fluor™ 6WS alkyne [TF6WS alkyne] | 682 | 701 | 220000 | 0.101 |
Tide Fluor™ 7WS alkyne [TF7WS alkyne] | 756 | 780 | 275000 | 0.049 |
Show More (2) |
Images

Figure 2. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Citations
View all 8 citations: Citation Explorer
Intraocular Delivery of a Collagen Mimetic Peptide Repairs Retinal Ganglion Cell Axons in Chronic and Acute Injury Models
Authors: Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J
Journal: International Journal of Molecular Sciences (2022): 2911
Authors: Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J
Journal: International Journal of Molecular Sciences (2022): 2911
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro
References
View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
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Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
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Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
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Journal: Antimicrob Agents Chemother (2009): 4311
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Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
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Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
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Rapid detection and quantification of Propionibacteriaceae
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Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
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Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
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Authors: Jothikumar P, Hill V, Narayanan J.
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