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Tide Fluor™ 2WS succinimidyl ester
TF2WS SE; Superior replacement for FITC
Tide Fluor™ 2WS (TF2WS) family has the spectral properties similar to those of fluorescein, DyLight 488 and Alexa Fluor 488. Compared to fluorescein probes TF2WS family has better water solubility. It has been used to label the hydrophobic peptides that hasve poor water solubility. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluorescein. In pairing with our Tide Quencher™ 2 (TQ2), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.
<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>
<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23495 mg
Price
 
Physical properties
Molecular weight945.07
SolventDMSO
Spectral properties
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025