Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1045.36 |
| Solvent | MeCN |
Spectral properties
| Absorbance (nm) | 560 |
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 560 |
| Emission (nm) | 580 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Bioconjugation, Dye to Peptide Conjugation, Cell Structures and Organelles, Nucleus, Chemical Reagents, Förster Resonance Energy Transfer (FRET), Peptide and Oligonucleotide Labeling, Custom Oligonucleotide Labeling, Digital PCR, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), ROX Reference Dye for Real-Time PCR, Tide Fluor Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 1045.36 | Absorbance (nm) 560 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 560 | Emission (nm) 580 |
Tide Fluor™ 3 (TF3) phosphoramidite is a high-performance alternative to Cy3 phosphoramidite for the in-synthesis labeling of oligonucleotides. TF3-labeled oligonucleotides share nearly identical fluorescence spectra to those labeled with Cy3, but the significantly larger quantum yield of TF3 produces a much brighter fluorescence signal with greater photostability than Cy3. When paired with our Tide Quencher™ 3 (TQ3), TF3 can be used to produce a variety of highly-sensitive FRET peptides and nucleotides for detecting protease activity as well as molecular beacons with enhanced sensitivity and stability.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 95.661 µL | 478.304 µL | 956.608 µL | 4.783 mL | 9.566 mL |
| 5 mM | 19.132 µL | 95.661 µL | 191.322 µL | 956.608 µL | 1.913 mL |
| 10 mM | 9.566 µL | 47.83 µL | 95.661 µL | 478.304 µL | 956.608 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 560 |
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 560 |
| Emission (nm) | 580 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| Tide Fluor™ 3 azide [TF3 azide] | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 3 alkyne [TF3 alkyne] | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3* | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 3 amine [TF3 amine] *Superior replacement for Cy3* | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* | 554 | 578 | 750001 | 0.179 |
| Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite] | - | - | 22000 | 0.091 |
Images
Figure 1. Chemical structure for Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite*.
Citations
View all 1 citations: Citation Explorer
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
References
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