Name: Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite*
Brand: AAT Bioquest
SKU: 2274
Price: 465 USD
Availability: InStock

Tide Fluor™ 3 phosphoramidite

TF3 CEP; Superior replacement to Cy3 phosphoramidite

Tide Fluor™ 3 (TF3) phosphoramidite is a high-performance alternative to Cy3 phosphoramidite for the in-synthesis labeling of oligonucleotides. TF3-labeled oligonucleotides share nearly identical fluorescence spectra to those labeled with Cy3, but the significantly larger quantum yield of TF3 produces a much brighter fluorescence signal with greater photostability than Cy3. When paired with our Tide Quencher™ 3 (TQ3), TF3 can be used to produce a variety of highly-sensitive FRET peptides and nucleotides for detecting protease activity as well as molecular beacons with enhanced sensitivity and stability.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2274	100 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.661 µL	478.304 µL	956.608 µL	4.783 mL	9.566 mL
5 mM	19.132 µL	95.661 µL	191.322 µL	956.608 µL	1.913 mL
10 mM	9.566 µL	47.83 µL	95.661 µL	478.304 µL	956.608 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1045.36 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation

Authors:

Perlenfein, Tyler J and Murphy, Regina M

Journal:

Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme

Authors:

Olea, Charles and Joyce, Gerald F

Journal:

Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia

Authors:

Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G

Journal:

American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Authors:

Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined

Journal:

ECS Transactions (2015): 9--16

Array of biodegradable microrafts for isolation and implantation of living, adherent cells

Authors:

Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L

Journal:

RSC advances (2013): 9264--9272

Physical properties

Molecular weight	1045.36
Solvent	MeCN

Spectral properties

Absorbance (nm)	560
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	560
Emission (nm)	580

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 17, 2025