Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red*
TF4 is designed to be a superior fluorophore alternative to ROX and Texas Red®. TF4 has (a). stronger fluorescence intensity; (b). higher conjugation yield; and (c). longer shelf life. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to ROX and Texas Red®. In pairing with our Tide Quencher™ 4 (TQ4), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.
![Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftide-fluor-4-succinimidyl-ester-tf4-se-superior-replacement-for-rox-and-texas-red%2Ffigure-for-tide-fluor-4-succinimidyl-ester-tf4-se-superior-replacement-for-rox-and-texas-red_n3Vjs.jpg&w=640&q=75)
![Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftide-fluor-4-succinimidyl-ester-tf4-se-superior-replacement-for-rox-and-texas-red%2Ffigure-for-tide-fluor-4-succinimidyl-ester-tf4-se-superior-replacement-for-rox-and-texas-red_n3Vjs.jpg&w=640&q=75)
![Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftide-fluor-4-succinimidyl-ester-tf4-se-superior-replacement-for-rox-and-texas-red%2Ffigure-for-tide-fluor-4-succinimidyl-ester-tf4-se-superior-replacement-for-rox-and-texas-red_n3Vjs.jpg&w=128&q=25)
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 151.577 µL | 757.886 µL | 1.516 mL | 7.579 mL | 15.158 mL |
5 mM | 30.315 µL | 151.577 µL | 303.154 µL | 1.516 mL | 3.032 mL |
10 mM | 15.158 µL | 75.789 µL | 151.577 µL | 757.886 µL | 1.516 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
Tide Fluor™ 1 succinimidyl ester [TF1 SE]*Superior replacement for EDANS* | 341 | 448 | 20000 | 0.187 |
Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
Tide Fluor™ 3 succinimidyl ester [TF3 SE]*Superior replacement for Cy3* | 554 | 578 | 750001 | 0.179 |
Tide Fluor™ 5WS succinimidyl ester [TF5WS SE]*Superior replacement for Cy5* | 649 | 664 | 250000 | 0.027 |
Tide Fluor™ 6WS succinimidyl ester [TF6WS SE]*Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.101 |
Tide Fluor™ 7WS, succinimidyl ester [TF7WS SE]*Superior replacement for Cy7* | 756 | 780 | 275000 | 0.049 |
Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission* | 785 | 801 | 250000 | 0.109 |
Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] *Superior replacement for Cy3* | 551 | 563 | 150000 | 0.079 |
Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] *Superior replacement for FITC* | 491 | 516 | 75000 | 0.11 |
Citations
View all 8 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on $\beta$-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): 21071--21082
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): 21071--21082
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311