Tide Fluor™ 5WS amine [TF5WS amine] *Superior replacement for Cy5*

Tide Fluor™ 5WS amine

TF5WS amine; Superior replacement for Cy5

Tide Fluor™ 5WS (TF5WS) family has the spectral properties essentially identical to those of Cy5. Compared to Cy5 probes TF5WS family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy5. TF5WS-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy5. In pairing with our Tide Quencher™ 5WS (TQ5WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF5WS product is primarily used for labeling the small molecules that contain a carbonyl group.

<p>Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2279	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 5WS amine [TF5WS amine] *Superior replacement for Cy5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	97.94 µL	489.702 µL	979.403 µL	4.897 mL	9.794 mL
5 mM	19.588 µL	97.94 µL	195.881 µL	979.403 µL	1.959 mL
10 mM	9.794 µL	48.97 µL	97.94 µL	489.702 µL	979.403 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1021.03 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation

Authors:

Perlenfein, Tyler J and Murphy, Regina M

Journal:

Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme

Authors:

Olea, Charles and Joyce, Gerald F

Journal:

Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia

Authors:

Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G

Journal:

American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Authors:

Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined

Journal:

ECS Transactions (2015): 9--16

Array of biodegradable microrafts for isolation and implantation of living, adherent cells

Authors:

Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L

Journal:

RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens

Authors:

Yang GP, Erdman DD, Tondella ML, Fields BS.

Journal:

J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe

Authors:

Andreoni A, Bondani M, Nardo L.

Journal:

Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer

Authors:

Ogawa M, Kosaka N, Choyke PL, Kobayashi H.

Journal:

Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Authors:

Kim GI, Kim KW, Oh MK, Sung YM.

Journal:

Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening

Authors:

Yu X, Sainz B, Jr., Uprichard SL.

Journal:

Antimicrob Agents Chemother (2009): 4311

Physical properties

Molecular weight	1021.03
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.027
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	649
Emission (nm)	663
Quantum yield	0.27 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025