Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 987.05 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.027 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 649 |
| Emission (nm) | 664 |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| Tide Fluor™ 5WS amine [TF5WS amine] *Superior replacement for Cy5* |
| Tide Fluor™ 5WS acid [TF5WS acid] *Superior replacement for Cy5* |
Related products
| Overview |  SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, Tide Fluor Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 987.05 | Correction Factor (280 nm) 0.027 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 649 | Emission (nm) 664 |
Tide Fluor™ 5WS (TF5WS) family has the spectral properties essentially identical to those of Cy5. Compared to Cy5 probes TF5WS family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy5. TF5WS-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy5. In pairing with our Tide Quencher™ 5WS (TQ5WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF5WS product is used for post-labeling of thiol-modified oligonucleotides and and cysteine-containing peptides.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 101.312 µL | 506.56 µL | 1.013 mL | 5.066 mL | 10.131 mL |
| 5 mM | 20.262 µL | 101.312 µL | 202.624 µL | 1.013 mL | 2.026 mL |
| 10 mM | 10.131 µL | 50.656 µL | 101.312 µL | 506.56 µL | 1.013 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.027 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 649 |
| Emission (nm) | 664 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS* | 341 | 448 | 20000 | 0.187 |
| Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
| Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 5WS azide [TF5WS azide] | 649 | 664 | 250000 | 0.027 |
| Tide Fluor™ 5WS alkyne [TF5WS alkyne] | 649 | 664 | 250000 | 0.027 |
| Tide Fluor™ 4 maleimide [TF4 maleimide] *Superior replacement for ROX and Texas Red* | 578 | 602 | 90000 | 0.436 |
| Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.101 |
| Tide Fluor™ 7WS maleimide [TF7WS maleimide] *Superior replacement for Cy7* | 756 | 780 | 275000 | 0.049 |
| Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission* | 785 | 801 | 250000 | 0.109 |
Show More (3) | ||||
Images
Figure 1. Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Citations
View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro
References
View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
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The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
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Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
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Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
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Rapid detection and quantification of Propionibacteriaceae
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Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
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Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519